PUBLICATION
Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq
- Authors
- Dhillon-Richardson, R.M., Haugan, A.K., Martik, M.L.
- ID
- ZDB-PUB-221126-4
- Date
- 2023
- Source
- Methods in molecular biology (Clifton, N.J.) 2599: 271282271-282 (Journal)
- Registered Authors
- Keywords
- Assay for transposase-accessible chromatin using sequencing (ATAC-seq), Embryonic Development, Enhancer screening, Fluorescence-activated cell sorting (FACS), Gene Regulatory Networks, Zebrafish
- MeSH Terms
-
- Chromatin Immunoprecipitation Sequencing*
- Animals
- Gene Regulatory Networks
- Embryonic Development
- Hunting
- Female
- Zebrafish*/genetics
- PubMed
- 36427156 Full text @ Meth. Mol. Biol.
Citation
Dhillon-Richardson, R.M., Haugan, A.K., Martik, M.L. (2023) Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq. Methods in molecular biology (Clifton, N.J.). 2599:271282271-282.
Abstract
Interactions between transcription factors and regulatory DNA can be described by gene regulatory networks. These networks provide a systems-level view of embryonic tissue development. Here, we describe a protocol for the isolation, identification, and experimental manipulation of tissue-specific cis-regulatory elements during zebrafish embryonic development using low-input ATAC-seq. With the methods described, genome-wide assessments of regulatory DNA in small populations of developing tissues can be identified, allowing for the construction of gene regulatory networks.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping