PUBLICATION
Rassf7a promotes spinal cord regeneration and controls spindle orientation in neural progenitor cells
- Authors
- Zhu, P., Zheng, P., Kong, X., Wang, S., Cao, M., Zhao, C.
- ID
- ZDB-PUB-221122-18
- Date
- 2022
- Source
- EMBO reports 24(1): e54984 (Journal)
- Registered Authors
- Zhao, Chengtian
- Keywords
- Rassf7a, asymmetric neurogenic division, regeneration, spinal cord injury, spindle orientation
- MeSH Terms
-
- Animals
- Axons/physiology
- Cell Cycle
- Mammals
- Nerve Regeneration/physiology
- Neural Stem Cells*/metabolism
- Neurogenesis
- Spinal Cord Injuries/genetics
- Spinal Cord Injuries/metabolism
- Spinal Cord Regeneration*
- Transcription Factors*
- Zebrafish/growth & development
- Zebrafish Proteins*/metabolism
- PubMed
- 36408859 Full text @ EMBO Rep.
Citation
Zhu, P., Zheng, P., Kong, X., Wang, S., Cao, M., Zhao, C. (2022) Rassf7a promotes spinal cord regeneration and controls spindle orientation in neural progenitor cells. EMBO reports. 24(1):e54984.
Abstract
Spinal cord injury (SCI) can cause long-lasting disability in mammals due to the lack of axonal regrowth together with the inability to reinitiate spinal neurogenesis at the injury site. Deciphering the mechanisms that regulate the proliferation and differentiation of neural progenitor cells is critical for understanding spinal neurogenesis after injury. Compared with mammals, zebrafish show a remarkable capability of spinal cord regeneration. Here, we show that Rassf7a, a member of the Ras-association domain family, promotes spinal cord regeneration after injury. Zebrafish larvae harboring a rassf7a mutation show spinal cord regeneration and spinal neurogenesis defects. Live imaging shows abnormal asymmetric neurogenic divisions and spindle orientation defects in mutant neural progenitor cells. In line with this, the expression of rassf7a is enriched in neural progenitor cells. Subcellular analysis shows that Rassf7a localizes to the centrosome and is essential for cell cycle progression. Our data indicate a role for Rassf7a in modulating spindle orientation and the proliferation of neural progenitor cells after spinal cord injury.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping