PUBLICATION
Using a zebrafish model to understand adherent-invasive Escherichia coli infection
- Authors
- Nag, D., Farr, D., Raychaudhuri, S., Withey, J.H.
- ID
- ZDB-PUB-221118-14
- Date
- 2022
- Source
- STAR protocols 3: 101810 (Journal)
- Registered Authors
- Keywords
- Immunology, Microbiology, Model organisms, Molecular biology
- MeSH Terms
-
- Animals
- Bacterial Adhesion
- Escherichia coli
- Escherichia coli Infections*/therapy
- Inflammation/microbiology
- Zebrafish*
- PubMed
- 36386878 Full text @ STAR Protoc
Citation
Nag, D., Farr, D., Raychaudhuri, S., Withey, J.H. (2022) Using a zebrafish model to understand adherent-invasive Escherichia coli infection. STAR protocols. 3:101810.
Abstract
A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequently, colonization and inflammation can be assessed. Exposing fish to probiotic at different time points relative to AIEC can determine beneficial effects of probiotics as prophylactics or therapeutics against AIEC. For complete details on the use and execution of this protocol, please refer to Nag et al. (2022).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping