PUBLICATION
The N-Substituted-4-Methylbenzenesulphonyl Hydrazone Inhibits Angiogenesis in Zebrafish Tg(fli1: EGFP) Model
- Authors
- Gawrońska-Grzywacz, M., Piątkowska-Chmiel, I., Popiołek, Ł., Herbet, M., Dudka, J.
- ID
- ZDB-PUB-221115-9
- Date
- 2022
- Source
- Pharmaceuticals (Basel, Switzerland) 15(11): (Journal)
- Registered Authors
- Keywords
- FET, Tg(fli1: EGFP), angiogenesis, sulphonyl hydrazone, zebrafish
- MeSH Terms
- none
- PubMed
- 36355480 Full text @ Pharmaceuticals (Basel)
Citation
Gawrońska-Grzywacz, M., Piątkowska-Chmiel, I., Popiołek, Ł., Herbet, M., Dudka, J. (2022) The N-Substituted-4-Methylbenzenesulphonyl Hydrazone Inhibits Angiogenesis in Zebrafish Tg(fli1: EGFP) Model. Pharmaceuticals (Basel, Switzerland). 15(11):.
Abstract
One of the most important therapies of malignant neoplasms, which are the second cause of death worldwide, is focused on the inhibition of pathological angiogenesis within the tumor. Therefore, the searching for the efficacious and relatively inexpensive small-molecule inhibitors of this process is essential. In this research, the anti-angiogenic potential of N-substituted-4-methylbenzenesulphonyl hydrazone, possessing antiproliferative activity against cancer cells, was tested. For this purpose, an intersegmental vessel (ISV) angiogenesis assay was performed using 6 hpf (hours post fertilization), 12 hpf and 24 hpf embryos of zebrafish transgenic strain, Tg(fli1: EGFP). They were incubated with different concentrations of tested molecule and after 24 h the development of intersegmental vessels of the trunk was analysed. In turn, the acute toxicity study in the zebrafish model was mainly conducted on strain AB, using the OECD-approved and recommended fish embryo acute toxicity test (FET) procedure. The results showed the moderate toxicity of N-[(3-chloro-4-methoxyphenyl)methylidene]-4-methylbenzenesulphonohydrazide in above-mentioned model with the LC50 value calculated at 23.04 mg/L. Moreover, newly synthesized molecule demonstrated the anti-angiogenic potential proved in Tg(fli1: EGFP) zebrafish model, which may be promising for the therapy of neoplastic tumors as well as other diseases related to pathological angiogenesis, such as age-related macular degeneration and diabetic retinopathy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping