PUBLICATION
Acertannin attenuates LPS-induced inflammation by interrupting the binding of LPS to the TLR4/MD2 complex and activating Nrf2-mediated HO-1 activation
- Authors
- Menu Neelaka Molagoda, I., Arachchilage Hasitha Maduranga Karunarathne, W., Lee, M.H., Kang, C.H., Tae Lee, K., Hyun Choi, Y., Lee, S., Kim, G.Y.
- ID
- ZDB-PUB-221025-7
- Date
- 2022
- Source
- International Immunopharmacology 113: 109344 (Journal)
- Registered Authors
- Keywords
- Acertannin, Anti-inflammation, ROS, TLR4/MD2
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/pharmacology
- Anti-Inflammatory Agents/therapeutic use
- Antioxidants/therapeutic use
- Heme Oxygenase-1*/metabolism
- Inflammation/chemically induced
- Inflammation/drug therapy
- Inflammation/metabolism
- Lipopolysaccharides*/pharmacology
- Molecular Docking Simulation
- NF-E2-Related Factor 2/metabolism
- NF-kappa B/metabolism
- Reactive Oxygen Species/metabolism
- Toll-Like Receptor 4/metabolism
- Zebrafish
- PubMed
- 36274481 Full text @ Int. Immunopharmacol.
Citation
Menu Neelaka Molagoda, I., Arachchilage Hasitha Maduranga Karunarathne, W., Lee, M.H., Kang, C.H., Tae Lee, K., Hyun Choi, Y., Lee, S., Kim, G.Y. (2022) Acertannin attenuates LPS-induced inflammation by interrupting the binding of LPS to the TLR4/MD2 complex and activating Nrf2-mediated HO-1 activation. International Immunopharmacology. 113:109344.
Abstract
Acertannin (ACTN) is a polyphenol known for its powerful anticancer and antioxidant effects. However, its anti-inflammatory effects have not been investigated at the molecular levels. Therefore, to evaluate anti-inflammatory effects of ACTN and its signaling pathway, the expression of proinflammatory markers was measured in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Molecular docking predicted the binding site of ACTN to the TLR4/MD2 complex. Moreover, in LPS-microinjected zebrafish, we investigated whether ACTN reduces nitric oxide and reactive oxygen species (ROS) production. ACTN significantly attenuated LPS-induced proinflammatory cytokines and mediators by inhibiting nuclear factor-kappa B (NF-κB) activation. ACTN also reduced LPS-induced ROS production and activated nuclear factor E2-related factor 2 and heme oxygenase-1 (HO-1). In addition, zinc protoporphyrin, an HO-1 inhibitor, markedly abolished the anti-inflammatory and antioxidant effects of ACTN in LPS-stimulated zebrafish larvae. Moreover, molecular docking predictions verified that ACTN forms a conventional hydrogen bond with LYS91 in myeloid differentiation factor-2 (MD2) and interrupts LPS binding to the Toll-like receptor 4 (TLR4)/MD2 complex. In addition, ACTN forms many non-covalent bonds, such as π-π stacking, π-alkyl, unfavorable donor-donor, and van der Waals interactions, with the TLR4/MD2 complex. Furthermore, the binding of ACTN to the TLR4/MD2 complex inhibited the recruitment of intracellular adaptor proteins, including myeloid differentiation primary response 88 and interleukin-1 receptor-associated kinase 4, and consequently attenuated NF-κB-mediated inflammatory responses. The conclusion of this study is that ACTN is a potent anti-inflammatory agent in LPS-mediated inflammation, such as endotoxemia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping