PUBLICATION
Chemo-kindling in adult zebrafish alters spatial cognitive functions but not social novelty recognition
- Authors
- Kumari, S., Dhiman, P., Kumar, R., Rahmatkar, S.N., Singh, D.
- ID
- ZDB-PUB-221018-82
- Date
- 2022
- Source
- Behavioural brain research 438: 114158 (Journal)
- Registered Authors
- Keywords
- Cognitive deficit, Dopamine, Epilepsy, HPLC-ECD, Pentylenetetrazole, Serotonin
- MeSH Terms
-
- Animals
- Anticonvulsants/pharmacology
- Cognition
- Epilepsy*/chemically induced
- Kindling, Neurologic*
- Pentylenetetrazole/pharmacology
- Zebrafish
- PubMed
- 36243243 Full text @ Behav. Brain Res.
Citation
Kumari, S., Dhiman, P., Kumar, R., Rahmatkar, S.N., Singh, D. (2022) Chemo-kindling in adult zebrafish alters spatial cognitive functions but not social novelty recognition. Behavioural brain research. 438:114158.
Abstract
In the past decades, zebrafish have gathered immense attention and importance in the field of neurological sciences. In the case of epilepsy, zebrafish have appeared as a promising acute animal model for the screening and identification of potential antiepileptic molecules. However, the necessity for establishing competent chronic models of epilepsy in zebrafish is apparent. In this regard, recently we developed a chemo-kindling zebrafish model with a better clinical resemblance. In the present study, an attempt to examine the effect of pentylenetetrazole (PTZ)-induced kindling on the cognitive functions of zebrafish was made. In brief, a number of fish were repetitively given a sub-effective concentration of PTZ, till the onset of clonic-tonic seizures, entitled as kindled. Thereafter, T-maze test and social recognition memory test to evaluate the spatial memory and social recognition memory were conducted in kindle fish groups. At the end, both the groups sacrificed and brains were isolated to estimate the alterations in the neurotransmitter and gene expression levels. It was observed that PTZ kindling induced spatial cognition deficits and lower social exploration in zebrafish. However, it didn't change the novelty recognition memory of kindled zebrafish. The results of genes and neurotransmitters estimations in the brain also supported the behavioural findings. The results concluded that PTZ kindling alters spatial cognitive functions in adult zebrafish without affecting the social novelty recognition memory.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping