PUBLICATION

Motilin is a regulator of gastric contraction in Japanese fire belly newts (Cynops pyrrhogaster), in vitro studies using isolated gastrointestinal strips of newts, rabbits, and chickens

Authors
Zhang, S., Kaiya, H., Kitazawa, T.
ID
ZDB-PUB-221018-46
Date
2022
Source
General and comparative endocrinology   330: 114140 (Journal)
Registered Authors
Keywords
Amphibian, Japanese fire belly newt, Urodelean, gastrointestinal contraction, motilin
MeSH Terms
  • Gastrointestinal Tract*/physiology
  • Stomach
  • Humans
  • Zebrafish
  • Salamandridae
  • Chickens
  • Rabbits
  • Animals
  • Motilin*/chemistry
  • Muscle Contraction*
  • Gastrointestinal Motility*
PubMed
36228737 Full text @ Gen. Comp. Endocrinol.
Abstract
The effects of newt motilin on the contractility of the isolated gastrointestinal (GI) tract from Japanese fire belly newts (newt) were examined to clarify whether motilin regulates GI motility in urodele amphibians. In addition, contractile responsiveness to motilins from seven species of vertebrates (human, chicken, turtle, alligator, axolotol, newt and zebrafish) were compared in GI preparations from three different animals (rabbit duodenum, chicken ileum and newt stomach) to determine the species-specific action of motilin. Newt motilin (10-10 M - 10-6 M) caused a contraction of cognate gastric strips, while the upper, middle, and lower intestinal strips were insensitive. The rank order of motilins for contractile activity in newt gastric strips was newt > alligator > axolotol > chicken > turtle > human >> zebrafish. On the other hand, newt motilin caused a weak contraction in the rabbit duodenum (human > alligator = chicken > turtle > axolotol ≧ newt > zebrafish), and it was ineffective in the chicken ileum (chicken > turtle = alligator > human >> newt, axolotol and zebrafish). This study demonstrates that motilin induces contraction in the GI tract of a urodele amphibian, the newt, in a region (stomach)-specific manner and further indicates that a ligand-receptor interaction of the motilin system is a species-specific manner probably due to differences in the amino acid sequence of motilin.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping