PUBLICATION

Mitochondrial network expansion and dynamic redistribution during islet morphogenesis in zebrafish larvae

Authors
Freudenblum, J., Meyer, D., Kimmel, R.A.
ID
ZDB-PUB-221018-25
Date
2022
Source
FEBS letters   597(2): 262-275 (Journal)
Registered Authors
Kimmel, Robin, Meyer, Dirk
Keywords
Mitochondria, image analysis, in vivo imaging, islet, pancreas, zebrafish
MeSH Terms
  • Cell Movement
  • Larva
  • Morphogenesis
  • Humans
  • Mitochondria*/metabolism
  • Animals
  • Zebrafish*/metabolism
(all 7)
PubMed
36217213 Full text @ FEBS Lett.
Abstract
Mitochondria, organelles critical for energy production, modify their shape and location in response to developmental state and metabolic demands. Mitochondria are altered in diabetes, but the mechanistic basis is poorly defined, due to difficulties in assessing mitochondria within an intact organism. Here, we use in vivo imaging in transparent zebrafish larvae to demonstrate filamentous, interconnected mitochondrial networks within islet cells. Mitochondrial movements highly resemble what has been reported for human islet cells in vitro, showing conservation in behavior across species and cellular context. During islet development, mitochondrial content increases with emergence of cell motility, and mitochondria disperse within fine protrusions. Overall, this work presents quantitative analysis of mitochondria within their native environment and provides insights into mitochondrial behavior during organogenesis.
Genes / Markers
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Expression
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Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b140
    		Unknown
    b692
      		Point Mutation
      cms1TgTransgenic Insertion
        ml10TgTransgenic Insertion
          ml80TgTransgenic Insertion
            ml202TgTransgenic Insertion
              ulg302TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
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                Sequence Targeting Reagents
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                Fish
                No data available
                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                DsRedEFGDsRed
                EGFPEFGEGFP
                GFPEFGGFP
                mCherryEFGmCherry
                mKate2EFGmKate2
                NTREFGNTR
                1 - 6 of 6
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                Citation
                Freudenblum, J., Meyer, D., Kimmel, R.A. (2022) Mitochondrial network expansion and dynamic redistribution during islet morphogenesis in zebrafish larvae. FEBS letters. 597(2):262-275.