PUBLICATION

Oral administration of hydroalcoholic extract of Centella asiatica ameliorates neurotoxicity and neurobehavioral deficits in 3-nitropropionic acid-induced Huntington's like symptoms in adult zebrafish

Authors
Kumar, V., Singh, C., Singh, A.
ID
ZDB-PUB-220924-37
Date
2022
Source
Rejuvenation Research   25(6): 260-274 (Journal)
Registered Authors
Keywords
3-nitropropionic acid, Centella asiatica, neuroinflammation zebrafish., neurotoxicity, oxidative stress
MeSH Terms
  • Animals
  • Oxidative Stress
  • Motor Activity
  • Pain/drug therapy
  • Plant Extracts/pharmacology
  • Plant Extracts/therapeutic use
  • Zebrafish/metabolism
  • Huntington Disease*/chemically induced
  • Huntington Disease*/drug therapy
  • Centella*/metabolism
  • Body Weight
  • Neuroprotective Agents*/pharmacology
  • Neuroprotective Agents*/therapeutic use
  • Glutathione/metabolism
  • Nitrites/adverse effects
  • Neuroinflammatory Diseases
  • Neurodegenerative Diseases*
(all 17)
PubMed
36150031 Full text @ Rejuvenation Res.
Abstract
Background Huntington's disease is an inherited neurodegenerative disease. 3- nitropropionic acid (3-NP) causes increased ROS production and neuroinflammation. Centella asiatica (CA) is a strong antioxidant.
Aim To investigate the effect of hydroalcoholic extract of Centella asiatica (HA-CA) in 3-nitro propionic acid-induced Huntington's disease in adult zebrafish.
Material and method Adult zebrafish (approximately 5-6 months old), weighing from 470-530 mg were used and treated with 3-NP (5 mg/kg i.p). The animals received HA-CA (80, and 100 mg/l) daily for up to twenty-eight days in water. Tetrabenazine (TBZ) (3 mg/kg i.p.) was used as standard drug. The body weight and behavior were performed before 3-NP administration on every 4th day. Then animals were sacrificed for biochemical, molecular, and histopathological analysis.
Results 3-NP treated zebrafish showed marked reduction in body weight, cognition, and locomotion activity accompanied by progressive neuronal damage. HA-CA treated group significantly reversed 3-NP induced increase level of LPO, AChEs, nitrite, and decrease the level of GSH. Administration of HA-CA (80, 100 mg/l) significantly ameliorates 3-NP induced alteration in body weight and behaviors and decreases neuroinflammation by decreasing TNF-α, and IL-1β levels in the brain. Moreover, HA-CA significantly decreases the 3-NP induced neuronal damage in the brain.
Conclusion HA-CA exhibits a protective effect against 3-NP HD-like symptoms in adult zebrafish due to anti-oxidant and anti-inflammatory mechanisms.
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Citation
Kumar, V., Singh, C., Singh, A. (2022) Oral administration of hydroalcoholic extract of Centella asiatica ameliorates neurotoxicity and neurobehavioral deficits in 3-nitropropionic acid-induced Huntington's like symptoms in adult zebrafish. Rejuvenation Research. 25(6):260-274.