PUBLICATION
Roles of Ca2+ activity in injury-induced migration of microglia in zebrafish in vivo
- Authors
- Du, T., Zhou, X., Zhang, R.D.Y., Du, X.F.
- ID
- ZDB-PUB-220921-11
- Date
- 2022
- Source
- Biochemistry and biophysics reports 32: 101340 (Journal)
- Registered Authors
- Xu-Fei, Du
- Keywords
- Ca2+ activity, IP3 receptor, Microglia, Migration, Two-photon imaging, Zebrafish
- MeSH Terms
- none
- PubMed
- 36120493 Full text @ Biochem Biophys Rep
Citation
Du, T., Zhou, X., Zhang, R.D.Y., Du, X.F. (2022) Roles of Ca2+ activity in injury-induced migration of microglia in zebrafish in vivo. Biochemistry and biophysics reports. 32:101340.
Abstract
Microglia are the resident immune cells in the brain. It is well known that brain injury can activate the microglia and induce its directional migration towards the injury sites for exerting immune functions. While extracellular ATP released from the injury site mediates the directionality of activated microglia's migration, what endows activated microglia with migration capability remains largely unexplored. In the present study, we used the larval zebrafish as an in vivo model to visualize the dynamics of both morphology and Ca2+ activity of microglia during its migration evoked by local brain injury. We found that, in response to local injury, activated microglia exhibited an immediate Ca2+ transient and later sustained Ca2+ bursts during its migration towards the local injury site. Furthermore, suppression of Ca2+ activities significantly retarded microglial cell migration. Thus, our study suggests that intracellular Ca2+ activity is required for activated microglia's migration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping