Optical Control of MicroRNA Function in Zebrafish Embryos
- Brown, W., Bardhan, A., Darrah, K., Tsang, M., Deiters, A.
- Journal of the American Chemical Society 144(37): 16819-16826 (Journal)
- Registered Authors
- Tsang, Michael
- MeSH Terms
- Embryo, Nonmammalian
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/pharmacology
- Zebrafish Proteins/genetics
- 36073798 Full text @ J. Am. Chem. Soc.
Brown, W., Bardhan, A., Darrah, K., Tsang, M., Deiters, A. (2022) Optical Control of MicroRNA Function in Zebrafish Embryos. Journal of the American Chemical Society. 144(37):16819-16826.
MicroRNAs play crucial and dynamic roles in vertebrate development and diseases. Some, like miR-430, are highly expressed during early embryo development and regulate hundreds of transcripts, which can make it difficult to study their role in the timing and location of specific developmental processes using conventional morpholino oligonucleotide (MO) knockdown or genetic deletion approaches. We demonstrate that light-activated circular morpholino oligonucleotides (cMOs) can be applied to the conditional control of microRNA function. We targeted miR-430 in zebrafish embryos to study its role in the development of the embryo body and the heart. Using 405 nm irradiation, precise spatial and temporal control over miR-430 function was demonstrated, offering insight into the cell populations and developmental timepoints involved in each process.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes