PUBLICATION

Optical Control of MicroRNA Function in Zebrafish Embryos

Authors
Brown, W., Bardhan, A., Darrah, K., Tsang, M., Deiters, A.
ID
ZDB-PUB-220909-2
Date
2022
Source
Journal of the American Chemical Society   144(37): 16819-16826 (Journal)
Registered Authors
Tsang, Michael
Keywords
none
MeSH Terms
  • Animals
  • Embryo, Nonmammalian
  • MicroRNAs*/genetics
  • Morpholinos/pharmacology
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/pharmacology
  • Zebrafish*
  • Zebrafish Proteins/genetics
PubMed
36073798 Full text @ J. Am. Chem. Soc.
Abstract
MicroRNAs play crucial and dynamic roles in vertebrate development and diseases. Some, like miR-430, are highly expressed during early embryo development and regulate hundreds of transcripts, which can make it difficult to study their role in the timing and location of specific developmental processes using conventional morpholino oligonucleotide (MO) knockdown or genetic deletion approaches. We demonstrate that light-activated circular morpholino oligonucleotides (cMOs) can be applied to the conditional control of microRNA function. We targeted miR-430 in zebrafish embryos to study its role in the development of the embryo body and the heart. Using 405 nm irradiation, precise spatial and temporal control over miR-430 function was demonstrated, offering insight into the cell populations and developmental timepoints involved in each process.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes