PUBLICATION
Zebrafish Danio rerio myotomal muscle structure and growth from a spatial transcriptomics perspective
- Authors
- Liu, G., Ito, T., Kijima, Y., Yoshitake, K., Asakawa, S., Watabe, S., Kinoshita, S.
- ID
- ZDB-PUB-220907-32
- Date
- 2022
- Source
- Genomics 114(5): 110477 (Journal)
- Registered Authors
- Kinoshita, Shigeharu, Watabe, Shugo
- Keywords
- Muscle growth, Muscle structure, Spatial transcriptomics, Zebrafish
- MeSH Terms
-
- Animals
- Larva
- Mammals
- Muscle Development/genetics
- Muscles
- Transcriptome*
- Zebrafish*/genetics
- PubMed
- 36058475 Full text @ Genomics
Citation
Liu, G., Ito, T., Kijima, Y., Yoshitake, K., Asakawa, S., Watabe, S., Kinoshita, S. (2022) Zebrafish Danio rerio myotomal muscle structure and growth from a spatial transcriptomics perspective. Genomics. 114(5):110477.
Abstract
Fish exhibit different muscle structures and growth characteristics compared with mammals. We used a spatial transcriptomics approach and examined myotomal muscle sections from zebrafish. Adult muscles were divided into eight regions according to spatial gene expression characteristics. Slow muscle was located in the wedge-shaped region near the lateral line and at the base of the dorsal fin, intermediate muscle was located in a ribbon-shaped region adjacent to slow muscle, and fast muscle was located in the deep region of the trunk, surrounded by intermediate muscle; the interior of fast muscle was further divided into 6 parts by their transcriptomic features. Combined analysis of adult and larval data revealed that adult muscles contain specific regions similar to larval muscles. These regions showed active myogenesis and a high expression of genes associated with muscle hyperplasia. This is the first study to apply spatial transcriptomics to fish myotomal muscle structure and growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping