PUBLICATION
CORRECTION: Treponema pallidum (syphilis) antigen TpF1 induces angiogenesis through the activation of the IL-8 pathway
- Authors
- Pozzobon, T., Facchinello, N., Bossi, F., Capitani, N., Benagiano, M., Di Benedetto, G., Zennaro, C., West, N., Codolo, G., Bernardini, M., Baldari, C.T., D'Elios, M.M., Pellegrini, L., Argenton, F., de Bernard, M.
- ID
- ZDB-PUB-220906-94
- Date
- 2018
- Source
- Scientific Reports 8: 46945 (Other)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 29449640 Full text @ Sci. Rep.
Citation
Pozzobon, T., Facchinello, N., Bossi, F., Capitani, N., Benagiano, M., Di Benedetto, G., Zennaro, C., West, N., Codolo, G., Bernardini, M., Baldari, C.T., D'Elios, M.M., Pellegrini, L., Argenton, F., de Bernard, M. (2018) CORRECTION: Treponema pallidum (syphilis) antigen TpF1 induces angiogenesis through the activation of the IL-8 pathway. Scientific Reports. 8:46945.
Abstract
This Article contains errors in the Materials and Methods section under subheading ‘RNA Isolation and RT-PCR in zebrafish’.
“One microgram of total RNA was reverse transcribed using MMLV reverse transcriptase and cDNA was amplified with the following primers: Danio rerio β-actin, 5′-CAGCAAGCAGGAGTACGATGAGT-3′ and 5′-TTGAATCTCATTGCTAGGCCATT-3′; Danio rerio IL-8a, 5′-AGCTTGAGAGGTCTGGCTGTAGA-3′ and 5′-CGAGGCGTTGATAAGCTCTCTGCT-3′, Danio rerio IL-8b, 5′-TGTTTTCCTGGCATTTCTGACC-3′and 5′-TTTACAGTGTGGGCT TGGAGGG-3′, Danio rerio VEGF-A, 5′-GATGTGATTCCCTTCATGGATGTGT-3′ and 5′-GGATACTCCTGGATG ATGTCTACCA-3′.”
should read:
“One microgram of total RNA was reverse transcribed using MMLV reverse transcriptase and cDNA was amplified with the following primers: Danio rerio VEGF-A, 5′-GATGTGATTCCCTTCATGGATGTGT-3′ and 5′-GGATACTCCTGGATGATGTCTACCA-3′; Danio rerio β-actin, 5′-CAGCAAGCAGGAGTACGATGAGT-3′ and 5′-TTGAATCTCATTGCTAGGCCATT-3′; for the IL-8 gene, amplification of two different fragments was performed with two couples of primers, hereafter be referred to as a and b: Danio rerio IL-8a, 5′-AGCTTGAGAGGTCTGGCTGTAGA-3′ and 5′-GCGTCGGCTTTCTGTTTCA-3′, Danio rerio IL-8b, 5′-TGTTTTCCTGGCATTTCTGACC-3′ and 5′-TTTACAGTGTGGGCTTGGAGGG-3′.”
“One microgram of total RNA was reverse transcribed using MMLV reverse transcriptase and cDNA was amplified with the following primers: Danio rerio β-actin, 5′-CAGCAAGCAGGAGTACGATGAGT-3′ and 5′-TTGAATCTCATTGCTAGGCCATT-3′; Danio rerio IL-8a, 5′-AGCTTGAGAGGTCTGGCTGTAGA-3′ and 5′-CGAGGCGTTGATAAGCTCTCTGCT-3′, Danio rerio IL-8b, 5′-TGTTTTCCTGGCATTTCTGACC-3′and 5′-TTTACAGTGTGGGCT TGGAGGG-3′, Danio rerio VEGF-A, 5′-GATGTGATTCCCTTCATGGATGTGT-3′ and 5′-GGATACTCCTGGATG ATGTCTACCA-3′.”
should read:
“One microgram of total RNA was reverse transcribed using MMLV reverse transcriptase and cDNA was amplified with the following primers: Danio rerio VEGF-A, 5′-GATGTGATTCCCTTCATGGATGTGT-3′ and 5′-GGATACTCCTGGATGATGTCTACCA-3′; Danio rerio β-actin, 5′-CAGCAAGCAGGAGTACGATGAGT-3′ and 5′-TTGAATCTCATTGCTAGGCCATT-3′; for the IL-8 gene, amplification of two different fragments was performed with two couples of primers, hereafter be referred to as a and b: Danio rerio IL-8a, 5′-AGCTTGAGAGGTCTGGCTGTAGA-3′ and 5′-GCGTCGGCTTTCTGTTTCA-3′, Danio rerio IL-8b, 5′-TGTTTTCCTGGCATTTCTGACC-3′ and 5′-TTTACAGTGTGGGCTTGGAGGG-3′.”
Errata / Notes
This article corrects ZDB-PUB-160106-9 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping