PUBLICATION

CORRECTION: Intronless WNT10B-short variant underlies new recurrent allele-specific rearrangement in acute myeloid leukaemia

Authors
Lazzaroni, F., Giacco, L.D., Biasci, D., Turrini, M., Prosperi, L., Brusamolino, R., Cairoli, R., Beghini, A.
ID
ZDB-PUB-220906-72
Date
2017
Source
Scientific Reports   7: 46788 (Other)
Registered Authors
Keywords
none
MeSH Terms
none
PubMed
28443613 Full text @ Sci. Rep.
Abstract
This Article contains typographical errors in the Methods section under subheading ?WNT10B/WNT10BIVS1 Gene expression analysis?.
?The WNT10B (P4-P2 primers) amplification was performed with following thermal conditions: 94?°C for 1?min, 33 cycles at 94° for 30?s, 58?°C for 30?s, 72?°C for 30?s and 72?°C for 5?min. The amplification of WNT10BIVS1 (P3-P2 primers) was performed as follows: 94?°C for 1?min, 33 cycles at 94° for 30?s, 61?°C for 30?s, 72?°C for 30?s and 72?°C for 5?min?.
should read:
?The WNT10B (P4-P1 primers) amplification was performed with following thermal conditions: 94?°C for 1?min, 33 cycles at 94° for 30?s, 58?°C for 30?s, 72?°C for 30?s and 72?°C for 5?min. The amplification of WNT10BIVS1 (P3-P1 primers) was performed as follows: 94?°C for 1?min, 33 cycles at 94° for 30?s, 61?°C for 30?s, 72?°C for 30?s and 72?°C for 5?min?.
In the same section, under subheading ?WNT10B/WNT10BIVS1 Absolute quantification?,
?We performed the experiment on Bio-Rad?s QX100 ddPCR system and the reaction mixtures in a final 20??l volume consisted of 10??l of 2?×?One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1?mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5??M of primers (WNT10B: P4-P2, WNT10BIVS1 P3-P2), 0.25??M WNT10B_dd1 and WNT10BIVS1_dd2 probes?.
should read:
?We performed the experiment on Bio-Rad?s QX100 ddPCR system and the reaction mixtures in a final 20??l volume consisted of 10??l of 2?×?One-Step RT-ddPCR Supermix (Bio-Rad, CA, USA), 1?mM Manganese Acetate solution (Bio-Rad, CA, USA), 0.5??M of primers (WNT10B: P4-P1, WNT10BIVS1 P3-P1), 0.25??M WNT10B_dd1 and WNT10BIVS1_dd2 probes?.
Errata / Notes
This article corrects ZDB-PUB-180518-9 .
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping