PUBLICATION
Exposure to leucine alters glutamate levels and leads to memory and social impairment in zebrafish
- Authors
- da Silva Lemos, I., Wessler, L.B., Duarte, M.B., da Silva, G.L., Bernardo, H.T., Candiotto, G., Torres, C.A., Petronilho, F., Rico, E.P., Streck, E.L.
- ID
- ZDB-PUB-220831-19
- Date
- 2022
- Source
- Metabolic Brain Disease 37(8): 2925-2935 (Journal)
- Registered Authors
- Keywords
- Alpha-branched-chain amino acids, Behavior, Glutamate, Maple syrup urine disease, Zebrafish
- MeSH Terms
-
- Animals
- Leucine
- Zebrafish*
- Maple Syrup Urine Disease*/metabolism
- Glutamic Acid
- Amino Acids, Branched-Chain/pharmacology
- PubMed
- 36040712 Full text @ Metab. Brain Dis.
Citation
da Silva Lemos, I., Wessler, L.B., Duarte, M.B., da Silva, G.L., Bernardo, H.T., Candiotto, G., Torres, C.A., Petronilho, F., Rico, E.P., Streck, E.L. (2022) Exposure to leucine alters glutamate levels and leads to memory and social impairment in zebrafish. Metabolic Brain Disease. 37(8):2925-2935.
Abstract
Maple Syrup Urine Disease (MSUD) is a metabolic disorder characterized by high levels in blood and urine of branched-chain amino acids leucine, isoleucine, and valine and their alpha-ketoacids, by a partial or total blockade in the activity of branched-chain complex alpha-keto acids dehydrogenase. The main symptoms in MSUD occur in the central nervous system, including cognitive deficits, locomotor, poor feeding, seizures, psychomotor delay, and mental retardation, but the mechanisms of neurotoxicity and behavior alteration due to this disease are poorly understood, thus this study aimed at showing the effects of leucine exposure on glutamate levels and behavior in zebrafish. For this, we analyzed the behavior using the social preference test and novel object recognition test, moreover, we analyse the glutamate levels and uptake using scintillation and high-performance liquid chromatography methods. Our results demonstrated a decrease in glutamate levels and uptake, accompanied by memory and social impairment. In conclusion, these results suggest that alterations in glutamate levels can be associated with behavior impairment, however, more studies are necessary to understand the mechanisms for brain damage in MSUD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping