PUBLICATION
Structure, anti-tumor activity, and potential anti-tumor mechanism of a fungus polysaccharide from Fomes officinalis
- Authors
- Zhang, S., Li, Y., Li, Z., Liu, W., Zhang, H., Ohizumi, Y., Nakajima, A., Xu, J., Guo, Y.
- ID
- ZDB-PUB-220824-5
- Date
- 2022
- Source
- Carbohydrate Polymers 295: 119794 (Journal)
- Registered Authors
- Keywords
- 2′,7′-Dichlorofluorescein diacetate (PubChem CID: 104913), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (PubChem CID: 64965), Acetic anhydride (PubChem CID: 7918), Angiogenesis, Anti-tumor mechanism, Dimethyl sulfoxide (PubChem CID: 679), Fomes officinalis Ames, Fungus polysaccharides, HepG2 cells, Lentinan (PubChem CID: 37723), Methyl iodide (PubChem CID: 6328), Propidium iodide (PubChem CID: 104981), Sodium borodeuteride (PubChem CID: 23673181), Tricaine (PubChem CID: 261501), Trifluoroacetic acid (PubChem CID: 6422), VEGF, Zebrafish
- MeSH Terms
-
- Animals
- Coriolaceae*
- Fungal Polysaccharides*/pharmacology
- Molecular Docking Simulation
- Polysaccharides/chemistry
- Polysaccharides/pharmacology
- Vascular Endothelial Growth Factor A
- Zebrafish
- PubMed
- 35988992 Full text @ Carbohydr. Polym.
Citation
Zhang, S., Li, Y., Li, Z., Liu, W., Zhang, H., Ohizumi, Y., Nakajima, A., Xu, J., Guo, Y. (2022) Structure, anti-tumor activity, and potential anti-tumor mechanism of a fungus polysaccharide from Fomes officinalis. Carbohydrate Polymers. 295:119794.
Abstract
In our ongoing process of discovering bioactive macromolecules, a homogeneous polysaccharide (FOP80-1) was first purified from Fomes officinalis. FOP80-1 with molecular weight of 4560 Da was mainly composed of →3)-d-Galp-(1→, →4)-β-d-Manp-(1→, →6)-α-d-Glcp-(1→, →3,6)-d-Glcp-(1→, and t--d-Glcp. Besides the structure features, the anti-tumor activity and potential mechanism of FOP80-1 were also investigated. The cellular and zebrafish experiments revealed that FOP80-1 inhibited tumor proliferation, invasion, and metastasis by increasing ROS, arresting cell cycle, inducing apoptosis, and suppressing angiogenesis. Corresponding to the inhibition of angiogenesis, the surface plasmon resonance (SPR) experiments revealed that FOP80-1 had good affinity with VEGF, a crucial protein to regulate angiogenesis. Molecular docking indicated that FOP80-1 could interact with the protein VEGF.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping