PUBLICATION

Bacterial-agglutinating and opsonic activities of RIPK1 in zebrafish

Authors
Duan, H., Yang, S., Zhang, X., Ji, G.
ID
ZDB-PUB-220819-12
Date
2022
Source
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   261: 109443 (Journal)
Registered Authors
Keywords
A pattern-recognition receptor, Lectin-like activity, RIPK1, Zebrafish
MeSH Terms
  • Animals
  • Gram-Negative Bacteria
  • Immunity, Innate
  • Lectins, C-Type
  • Lipopolysaccharides*/pharmacology
  • Zebrafish*
PubMed
35981661 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Abstract
Many studies have demonstrated that receptor interacting protein kinase-1 acts as a crucial mediator in the regulation of immune response, but evidence remains lacking for its direct interaction with bacteria. In this study, we found that challenge with lipopolysaccharide and lipoteichoic acid resulted in a significantly increased transcriptional expression of receptor interacting protein kinase-1 in zebrafish, suggesting the receptor interacting protein kinase-1 is implicated in anti-infectious responses. In accordance, we found that recombinant receptor interacting protein kinase-1 was not only able to bind to Gram-negative and -positive bacteria via interaction with lipopolysaccharide and lipoteichoic acid, but also agglutinate both Gram-negative and -positive bacteria in a Ca2+-dependent manner.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping