PUBLICATION

CRMP2 and CRMP4 are required for the formation of commissural tracts in the developing zebrafish forebrain

Authors
Guo, Y., Oliveros, C.F., Ohshima, T.
ID
ZDB-PUB-220806-6
Date
2022
Source
Developmental Neurobiology   82(6): 533-544 (Journal)
Registered Authors
Ohshima, Toshio
Keywords
Anterior commissure, Axon guidance, CRMP2, CRMP4, Forebrain, Neuropilin 1a, Postoptic commissure, Semaphorin 3, Zebrafish
MeSH Terms
  • Animals
  • Morpholinos/metabolism
  • Morpholinos/pharmacology
  • Neuropilins/metabolism
  • Prosencephalon/metabolism
  • RNA, Messenger/metabolism
  • Semaphorin-3A/metabolism
  • Semaphorins*/genetics
  • Semaphorins*/metabolism
  • Zebrafish*/metabolism
PubMed
35929227 Full text @ Dev. Neurobiol.
Abstract
Axonal connections between the two sides of the brain are essential for processing sensorimotor functions, especially in animals with bilateral symmetry. The anterior commissure and post-optic commissure are two crucial axonal projections that develop early in the zebrafish central nervous system. In this study, we characterized the function of collapsin response mediator protein 2 (CRMP2) and CRMP4 in patterning the development of the anterior and post-optic commissures by analyzing morpholino-knockdown zebrafish morphants and CRISPR/Cas9-edited gene-knockout mutants. We observed a loss of commissural structures or a significant reduction in axon bundles connecting the two hemispheres, but the defects could be largely recovered by co-injecting CRMP2 or CRMP4 mRNA. Loss of both CRMP2 and CRMP4 function resulted in a synergistic increase in the number of commissural defects. To elucidate the mechanism by which CRMP2 and CRMP4 provide guidance cues for the development of the anterior and post-optic commissures, we included neuropilin 1a (Nrp1a) morphants and double morphants (CRMP2/Nrp1a and CRMP4/Nrp1a) for analysis. Our experimental results indicated that CRMP2 and CRMP4 might mediate their activities through the common semaphorin 3/Nrp1a signaling pathway. This article is protected by copyright. All rights reserved.
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