PUBLICATION
A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish
- Authors
- Jang, J.Y., Liang, T., Kim, M.K., Kang, K.W., Lee, B., Choi, S.Y.
- ID
- ZDB-PUB-220726-33
- Date
- 2022
- Source
- Zebrafish 19(4): 160-164 (Journal)
- Registered Authors
- Choi, Seok-Yong
- Keywords
- Q-tips, extraction, genomic DNA, genotyping, swabbing, zebrafish
- MeSH Terms
-
- Animals
- DNA*/analysis
- Genomics
- Polymerase Chain Reaction/methods
- Sequence Analysis, DNA/methods
- Zebrafish*/genetics
- PubMed
- 35877315 Full text @ Zebrafish
Citation
Jang, J.Y., Liang, T., Kim, M.K., Kang, K.W., Lee, B., Choi, S.Y. (2022) A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish. Zebrafish. 19(4):160-164.
Abstract
Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from clipped caudal fin or skin mucus through either alkaline lysis using NaOH or proteinase K (PK) treatment. Further purification of the gDNA using ethanol precipitation was optional. To develop a rapid and noninvasive method that extracts PCR-ready gDNA from adult zebrafish, we combined skin swabbing with PK treatment and demonstrated its efficiency. This method could be applied to a wide range of fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping