PUBLICATION

A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish

Authors
Jang, J.Y., Liang, T., Kim, M.K., Kang, K.W., Lee, B., Choi, S.Y.
ID
ZDB-PUB-220726-33
Date
2022
Source
Zebrafish   19(4): 160-164 (Journal)
Registered Authors
Choi, Seok-Yong
Keywords
Q-tips, extraction, genomic DNA, genotyping, swabbing, zebrafish
MeSH Terms
  • Animals
  • DNA*/analysis
  • Genomics
  • Polymerase Chain Reaction/methods
  • Sequence Analysis, DNA/methods
  • Zebrafish*/genetics
PubMed
35877315 Full text @ Zebrafish
Abstract
Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from clipped caudal fin or skin mucus through either alkaline lysis using NaOH or proteinase K (PK) treatment. Further purification of the gDNA using ethanol precipitation was optional. To develop a rapid and noninvasive method that extracts PCR-ready gDNA from adult zebrafish, we combined skin swabbing with PK treatment and demonstrated its efficiency. This method could be applied to a wide range of fish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping