PUBLICATION
Temporal-spatial low shear stress induces heterogenous distribution of hematopoietic stem cell budding in zebrafish
- Authors
- Cui, Y., Shi, W., Zhang, K., Hou, Z., Wang, Y., Yan, W., Ma, Q., He, S., Huang, J., Lu, C., Wang, Y., Wang, G., Qiu, J.
- ID
- ZDB-PUB-220707-7
- Date
- 2022
- Source
- Cellular and molecular life sciences : CMLS 79: 399 (Journal)
- Registered Authors
- Cui, Yuliang, Qiu, Juhui, Wang, Guixue, Wang, Yeqi
- Keywords
- Blood flow, Hematopoietic stem/progenitor cells (HSPCs), Heterogeneity, Macrophage, Matrix metalloproteinases (MMPs)
- MeSH Terms
-
- Animals
- Endothelial Cells*/metabolism
- Hematopoiesis
- Hematopoietic Stem Cells/metabolism
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 35792959 Full text @ Cell. Mol. Life Sci.
Citation
Cui, Y., Shi, W., Zhang, K., Hou, Z., Wang, Y., Yan, W., Ma, Q., He, S., Huang, J., Lu, C., Wang, Y., Wang, G., Qiu, J. (2022) Temporal-spatial low shear stress induces heterogenous distribution of hematopoietic stem cell budding in zebrafish. Cellular and molecular life sciences : CMLS. 79:399.
Abstract
Hematopoietic stem/progenitor cells (HSPCs) originate from endothelial cells (ECs) localized on the ventral side of the dorsal aorta (DA), and hemodynamic parameters may suffer sharp changes in DA at HSPCs development stage for intersegmental vessel formation. However, the temporal-spatial shear stress parameters and biomechanics mechanisms of HSPC budding remain unknown. Here, we found that the hematopoietic endothelium (HE) in the aorta-gonad-mesonephros was heterogeneous; that is, HEs were mainly distributed at the ventral side of the vascular bifurcation in zebrafish embryos, which was found to show low shear stress (LSS) through numerical simulation analysis. Furthermore, HSPCs localized in the posterior somite of aorta-gonad-mesonephros with slow velocity. On the temporal scale, there was a slow velocity and LSS during HE budding from 36 h post-fertilization and decreased shear stress with drug expanded HSPC numbers. Mechanistically, matrix metalloproteinase (MMP) expression and macrophage chemotaxis were significantly increased in HEs by RNA-seq. After treatment with an MMP13 inhibitor, HSPCs were significantly reduced in both the aorta-gonad-mesonephros and caudal hematopoietic tissue in embryos. Our results show that HSPC budding is heterogeneous, and the mechanism is that physiological LSS controls the emergence of HSPCs by promoting the accumulation of macrophages and subsequent MMP expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping