PUBLICATION
Th1-Th2 and M1-M2 interplay sculpt Aeromonas hydrophila pathogenesis in zebrafish (Danio rerio)
- Authors
- Kumar, J., Kumar, M., Sharma, S., Srivastava, N., Singh, R., Hussain, M.A., Mazumder, S.
- ID
- ZDB-PUB-220701-14
- Date
- 2022
- Source
- Fish & shellfish immunology 127: 357-365 (Journal)
- Registered Authors
- Keywords
- Aeromonas hydrophila, Immune response, Immunized, M1-M2, Motile aeromonad septicemia, Pathogenesis, Th1–Th2, Zebrafish
- MeSH Terms
-
- Aeromonas hydrophila/physiology
- Animals
- Fish Diseases*
- Gram-Negative Bacterial Infections*
- Virulence
- Zebrafish
- PubMed
- 35772676 Full text @ Fish Shellfish Immunol.
Citation
Kumar, J., Kumar, M., Sharma, S., Srivastava, N., Singh, R., Hussain, M.A., Mazumder, S. (2022) Th1-Th2 and M1-M2 interplay sculpt Aeromonas hydrophila pathogenesis in zebrafish (Danio rerio). Fish & shellfish immunology. 127:357-365.
Abstract
Aeromonas hydrophila is an important aquatic zoonotic pathogen that causes septicemia, necrotizing fasciitis and gastroenteritis in various aquatic and non-aquatic animals. However, the pathogenesis of A. hydrophila is not fully understood. Here, we examined the pathogenicity and histopathology of A. hydrophila in the zebrafish (Danio rerio) model system. We found that the intensity of symptoms and mortality is dose-dependent. Bacterial colonization studies demonstrated that A. hydrophila never cleared out from the fish body but stayed in a state of inactivity till it enters a fresh host. Reinfection studies showed that exposure to A. hydrophila provides immunity against future infection and hence improves fish survival. Gene expression studies revealed the crosstalk between T-helper cell and macrophage responses in fish immune system in response to A. hydrophila and infection memory. Histopathological studies showed that symptoms of tissue damage and inflammation lasted for less duration with less intensity in immunized fish when compared to non-immunized fish. Together, our results suggest that the zebrafish model is a useful system in studying the interplay between A. hydrophila pathogenesis, persistence and immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping