PUBLICATION

Single-cell RNA-sequencing of zebrafish hair cells reveals novel genes potentially involved in hearing loss

Authors
Qian, F., Wei, G., Gao, Y., Wang, X., Gong, J., Guo, C., Wang, X., Zhang, X., Zhao, J., Wang, C., Xu, M., Hu, Y., Yin, G., Kang, J., Chai, R., Xie, G., Liu, D.
ID
ZDB-PUB-220628-1
Date
2022
Source
Cellular and molecular life sciences : CMLS   79: 385 (Journal)
Registered Authors
Gong, Jie, Liu, Dong, Wang, Xin
Keywords
Hair cell, Zebrafish, scRNA-seq
Datasets
GEO:GSE221471
MeSH Terms
  • Animals
  • Hair Cells, Auditory
  • Hearing Loss*
  • Mammals/genetics
  • RNA/metabolism
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
35753015 Full text @ Cell. Mol. Life Sci.
Abstract
Hair cells play key roles in hearing and balance, and hair cell loss would result in hearing loss or vestibular dysfunction. Cellular and molecular research in hair cell biology provides us a better understanding of hearing and deafness. Zebrafish, owing to their hair cell-enriched organs, have been widely applied in hair cell-related research worldwide. Similar to mammals, zebrafish have inner ear hair cells. In addition, they also have lateral line neuromast hair cells. These different types of hair cells vary in morphology and function. However, systematic analysis of their molecular characteristics remains lacking. In this study, we analyzed the GFP+ cells isolated from Tg(Brn3c:mGFP) larvae with GFP expression in all hair cells using single-cell RNA-sequencing (scRNA-seq). Three subtypes of hair cells, namely macula hair cell (MHC), crista hair cell (CHC), and neuromast hair cell (NHC), were characterized and validated by whole-mount in situ hybridization analysis of marker genes. The hair cell scRNA-seq data revealed hair cell-specific genes, including hearing loss genes that have been identified in humans and novel genes potentially involved in hair cell formation and function. Two novel genes were discovered to specifically function in NHCs and MHCs, corresponding to their specific expression in NHCs and MHCs. This study allows us to understand the specific genes in hair cell subpopulations of zebrafish, which will shed light on the genetics of both human vestibular and cochlear hair cell function.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping