PUBLICATION
The embryonic zebrafish brain is seeded by a lymphatic-dependent population of mrc1+ microglia precursors
- Authors
- Green, L.A., O'Dea, M.R., Hoover, C.A., DeSantis, D.F., Smith, C.J.
- ID
- ZDB-PUB-220622-17
- Date
- 2022
- Source
- Nature Neuroscience 25(7): 849-864 (Journal)
- Registered Authors
- Smith, Cody
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/physiology
- Humans
- Microglia*/metabolism
- Yolk Sac/metabolism
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 35710983 Full text @ Nat. Neurosci.
Citation
Green, L.A., O'Dea, M.R., Hoover, C.A., DeSantis, D.F., Smith, C.J. (2022) The embryonic zebrafish brain is seeded by a lymphatic-dependent population of mrc1+ microglia precursors. Nature Neuroscience. 25(7):849-864.
Abstract
Microglia are the resident macrophages of the CNS that serve critical roles in brain construction. Although human brains contain microglia by 4 weeks gestation, an understanding of the earliest microglia that seed the brain during its development remains unresolved. Using time-lapse imaging in zebrafish, we discovered a mrc1a+ microglia precursor population that seeds the brain before traditionally described microglia. These early microglia precursors are dependent on lymphatic vasculature that surrounds the brain and are independent of pu1+ yolk sac-derived microglia. Single-cell RNA-sequencing datasets reveal Mrc1+ microglia in the embryonic brains of mice and humans. We then show in zebrafish that these early mrc1a+ microglia precursors preferentially expand during pathophysiological states in development. Taken together, our results identify a critical role of lymphatics in the microglia precursors that seed the early embryonic brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping