PUBLICATION
Fatty Acid Incorporation in the Muscle, Oxidative Markers, Lipid Peroxidation and PPAR-α and SREBP-2 Expression of Zebrafish Fed Linseed Oil and Clove Leaf Essential Oil
- Authors
- Silva, T.C.D., Utsunomiya, K.S., Castro, P.L., Rocha, J.D.M., Visentainer, J.V., Gasparino, E., Ribeiro, R.P.
- ID
- ZDB-PUB-220616-5
- Date
- 2022
- Source
- Anais da Academia Brasileira de Ciencias 94: e20210236 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Syzygium*
- Zebrafish/metabolism
- PPAR alpha/analysis
- PPAR alpha/metabolism
- Fatty Acids/analysis
- Oxidative Stress
- Animals
- Plant Leaves/metabolism
- Linseed Oil/chemistry
- Linseed Oil/metabolism
- Linseed Oil/pharmacology
- Liver/metabolism
- Fatty Acids, Omega-3*/analysis
- Fatty Acids, Omega-3*/metabolism
- Sterol Regulatory Element Binding Protein 1/analysis
- Sterol Regulatory Element Binding Protein 1/metabolism
- Lipid Peroxidation
- Oils, Volatile*/metabolism
- Muscles/metabolism
- PubMed
- 35703694 Full text @ An. Acad. Bras. Cienc.
Citation
Silva, T.C.D., Utsunomiya, K.S., Castro, P.L., Rocha, J.D.M., Visentainer, J.V., Gasparino, E., Ribeiro, R.P. (2022) Fatty Acid Incorporation in the Muscle, Oxidative Markers, Lipid Peroxidation and PPAR-α and SREBP-2 Expression of Zebrafish Fed Linseed Oil and Clove Leaf Essential Oil. Anais da Academia Brasileira de Ciencias. 94:e20210236.
Abstract
The objective of this study is to assess, in zebrafish, the effects of combining linseed oil (LO) and clove leaf essential oil (CLEO) on the incorporation of fatty acids in the muscle, oxidative markers, lipid peroxidation and expression of the PPAR-α (Peroxisome Proliferator-Activated Receptor-α) and the SREBP-2 (Sterol Regulatory Element Binding Protein-2) genes. Six diets were prepared, containing combinations of LO (3, 6 and 9%) and CLEO (0.5 and 1%): 3% LO + 0.5% CLEO; 3% LO + 1% CLEO; 6% LO + 0.5% CLEO; 6% LO + 1% CLEO; 9% LO + 0.5% CLEO; 9% LO + 1% CLEO. Results showed increase in the incorporation of n-3 fatty acids in the muscle concomitantly with the addition of LO and CLEO. The activities of superoxide dismutase and catalase were reduced and the glutathione content had increased. Lipid peroxidation was lower in the treatment with 1% CLEO, regardless of LO content. The expression of the PPAR-α and the SREBP-2 genes was higher in animals fed 9% LO + 0.5% CLEO. Therefore, for a greater incorporation and protection against the oxidative damages of n-3 fatty acids, a combined use of 9% LO with 0.5% CLEO is recommended for zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping