PUBLICATION

Expression analysis of nel during zebrafish embryonic development

Authors
Zhao, J., Wei, G., Zhu, J., Liu, D., Qin, B.
ID
ZDB-PUB-220614-1
Date
2022
Source
Gene expression patterns : GEP   45: 119258 (Journal)
Registered Authors
Liu, Dong
Keywords
Development, Expression, Nel, Zebrafish
MeSH Terms
  • Animals
  • Central Nervous System/metabolism
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental
  • Phylogeny
  • Retina/metabolism
  • Zebrafish*
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
PubMed
35691514 Full text @ Gene Expr. Patterns
Abstract
Nel is a multimeric extracellular glycoprotein which predominantly expressed in the nervous system and play an important role in neural development and functions. There are three nel paralogues included nell2a, nell2b, and nell3 in zebrafish, while systematic expression analysis of the nel family is still lacking. In this study, we performed a phylogenetic analysis on 7 species, in different species the nell2a are highly conserved, as is nell2b. Then, the expression profiles of nell2a, nell2b and nell3 were detected by in situ hybridization in zebrafish embryo, and the result showed that nel genes highly enriched in the central nervous system, but distributed in different regions of the brain. In addition, nell2a is also expressed in the olfactory pit, spinal cord, otic vesicle and retina (ganglion cell layer), nell2b was detected to express in gill arches, olfactory epithelium, olfactory pit, spinal cord, photoreceptor and retina (ganglion cell layer), it should be noted that the expression of nell3 is special, was only detected at 96 hpf in the brain and spinal cord of zebrafish. Overall, our results indicate that nell2a and nell2b genes are expressed in the nervous system and eyes of zebrafish embryo, while nell3 is expressed in different regions in the nervous system. The phylogenetic analysis also shows that nell3 sequences are significantly different from nell2a and nell2b. This study provides new evidence to better understand the role of nel in zebrafish embryo development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping