PUBLICATION
Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle
- Authors
- Sugasawa, T., Komine, R., Manevich, L., Tamai, S., Takekoshi, K., Kanki, Y.
- ID
- ZDB-PUB-220611-9
- Date
- 2022
- Source
- Nutrients 14(11): (Journal)
- Registered Authors
- Keywords
- fasting, gene length, mRNA-sequencing, refeeding, skeletal muscle, zebrafish
- Datasets
- GEO:GSE201273
- MeSH Terms
-
- Animals
- Fasting*/physiology
- Muscle, Skeletal/metabolism
- RNA, Messenger/metabolism
- Transcriptome
- Zebrafish*/genetics
- PubMed
- 35684038 Full text @ Nutrients
Citation
Sugasawa, T., Komine, R., Manevich, L., Tamai, S., Takekoshi, K., Kanki, Y. (2022) Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle. Nutrients. 14(11).
Abstract
Recently, fasting has been spotlighted from a healthcare perspective. However, the de-tailed biological mechanisms and significance by which the effects of fasting confer health benefits are not yet clear. Due to certain advantages of the zebrafish as a vertebrate model, it is widely utilized in biological studies. However, the biological responses to nutrient metabolism within zebrafish skeletal muscles have not yet been amply reported. Therefore, we aimed to reveal a gene expression profile in zebrafish skeletal muscles in response to fasting-refeeding. Accordingly, mRNA-sequencing and bioinformatics analysis were performed to examine comprehensive gene expression changes in skeletal muscle tissues during fasting-refeeding. Our results produced a novel set of nutrition-related genes under a fasting-refeeding protocol. Moreover, we found that five genes were dramatically upregulated in each fasting (for 24 h) and refeeding (after 3 h), exhibiting a rapid response to the provided conditional changes. The assessment of the gene length revealed that the gene set whose expression was elevated only after 3 h of refeeding had a shorter length, suggesting that nutrition-related gene function is associated with gene length. Taken together, our results from the bioinformatics analyses provide new insights into biological mechanisms induced by fasting-refeeding conditions within zebrafish skeletal muscle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping