PUBLICATION
"Recording synaptic transmission from auditory mixed synapses on the Mauthner cells of developing zebrafish"
- Authors
- Echeverry, F.A., Ijaz, S., Pereda, A.E.
- ID
- ZDB-PUB-220601-17
- Date
- 2022
- Source
- eNeuro 9(3): (Journal)
- Registered Authors
- Keywords
- auditory afferent, connexin, electrical synapse, gap junction, glutamate, mixed transmission
- MeSH Terms
-
- Animals
- Gap Junctions/physiology
- Neurons/physiology
- Synapses/physiology
- Synaptic Transmission*/physiology
- Zebrafish*
- PubMed
- 35641226 Full text @ eNeuro
Citation
Echeverry, F.A., Ijaz, S., Pereda, A.E. (2022) "Recording synaptic transmission from auditory mixed synapses on the Mauthner cells of developing zebrafish". eNeuro. 9(3):.
Abstract
The Mauthner cells are a pair of large reticulospinal neurons that organize sensory-evoked tail flip responses in fishes. An identifiable group of auditory "mixed" (electrical and chemical) synaptic contacts known as "Large Myelinated Club Endings" on these cells have provided a valuable model for the study of synaptic transmission in the vertebrate brain. While most of studies were performed in adult fish, we describe here methods that make possible recording synaptic transmission from these contacts in developing zebrafish, a genetically tractable vertebrate species which is uniquely amenable for combining synaptic physiology with live imaging and behavioral analysis.Significance statementAn identifiable type of "mixed" synaptic contacts on the fish Mauthner cells provide a valuable model for the study of synaptic transmission in the vertebrate brain. We describe here methods to record synaptic potentials from these contacts in developing zebrafish. The approach makes possible to explore the molecular and functional properties of these model mixed synapses in a genetically tractable species at which synaptic mechanisms and their circuit functions can be more easily examined.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping