PUBLICATION

High glucose-induced ROS-accumulation in embryo-larval stages of zebrafish leads to mitochondria-mediated apoptosis

Authors
Li, Y., Chen, Q., Liu, Y., Bi, L., Jin, L., Xu, K., Peng, R.
ID
ZDB-PUB-220522-3
Date
2022
Source
Apoptosis : an international journal on programmed cell death   27(7-8): 509-520 (Journal)
Registered Authors
Keywords
Apoptosis, Diabetes, Mitochondria, ROS, Zebrafish
MeSH Terms
  • Animals
  • Apoptosis*
  • Embryo, Nonmammalian/metabolism
  • Glucose/metabolism
  • Glucose/pharmacology
  • Humans
  • Larva/metabolism
  • Mitochondria/metabolism
  • Oxidative Stress
  • Reactive Oxygen Species/metabolism
  • Zebrafish*
PubMed
35596834 Full text @ Apoptosis
Abstract
In recent decades, diabetes mellitus has become a major chronic disease threatening human health worldwide, and the age of patients tends to be younger; however, the pathogenesis remains unclear, resulting in many difficulties in its treatment. As an ideal model animal, zebrafish can simulate the processes of human diabetes well. In this study, we successfully established a model of diabetic zebrafish larvae in a previous work. Furthermore, transcriptome analysis was completed, and the results suggested that 10.59% of differentially expressed genes (DEGs) related to the apoptosis pathway need to be considered. Then, glucose-induced developmental toxicity, reactive oxygen species (ROS) accumulation, antioxidant system function, apoptosis and mitochondrial dysfunction were measured in zebrafish larvae. We hope that this study will provide valuable reference information for type 2 juvenile diabetes treatment.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping