PUBLICATION

Using Light-Sheet Microscopy to Study Spontaneous Activity in the Developing Lateral-Line System

Authors
Zhang, Q., Kindt, K.S.
ID
ZDB-PUB-220521-5
Date
2022
Source
Frontiers in cell and developmental biology   10: 819612 (Journal)
Registered Authors
Kindt, Katie, Zhang, Qiuxiang
Keywords
development, hair cell, lateral line efferents, light-sheet microscopy, spontaneous activity
MeSH Terms
none
PubMed
35592245 Full text @ Front Cell Dev Biol
Abstract
Hair cells are the sensory receptors in the auditory and vestibular systems of all vertebrates, and in the lateral-line system of aquatic vertebrates. The purpose of this work is to explore the zebrafish lateral-line system as a model to study and understand spontaneous activity in vivo. Our work applies genetically encoded calcium indicators along with light-sheet fluorescence microscopy to visualize spontaneous calcium activity in the developing lateral-line system. Consistent with our previous work, we show that spontaneous calcium activity is present in developing lateral-line hair cells. We now show that supporting cells that surround hair cells, and cholinergic efferent terminals that directly contact hair cells are also spontaneously active. Using two-color functional imaging we demonstrate that spontaneous activity in hair cells does not correlate with activity in either supporting cells or cholinergic terminals. We find that during lateral-line development, hair cells autonomously generate spontaneous events. Using localized calcium indicators, we show that within hair cells, spontaneous calcium activity occurs in two distinct domains-the mechanosensory bundle and the presynapse. Further, spontaneous activity in the mechanosensory bundle ultimately drives spontaneous calcium influx at the presynapse. Comprehensively, our results indicate that in developing lateral-line hair cells, autonomously generated spontaneous activity originates with spontaneous mechanosensory events.
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