PUBLICATION
Effects of di-(2-ethylhexyl) phthalate (DEHP) on behavior and dopamine signaling in zebrafish (Danio rerio)
- Authors
- Huang, W., Xiao, J., Shi, X., Zheng, S., Li, H., Liu, C., Wu, K.
- ID
- ZDB-PUB-220521-12
- Date
- 2022
- Source
- Environmental Toxicology and Pharmacology 93: 103885 (Journal)
- Registered Authors
- Wu, Kusheng
- Keywords
- Apoptosis, Di-(2-ethylhexyl) phthalate (DEHP), Dopamine signaling, Neurotoxicity, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Diethylhexyl Phthalate*/toxicity
- Dopamine/metabolism
- Larva
- Oxidative Stress
- Phthalic Acids
- Plasticizers/toxicity
- Superoxide Dismutase-1
- Zebrafish*/metabolism
- PubMed
- 35595013 Full text @ Environ. Toxicol. Pharmacol.
Citation
Huang, W., Xiao, J., Shi, X., Zheng, S., Li, H., Liu, C., Wu, K. (2022) Effects of di-(2-ethylhexyl) phthalate (DEHP) on behavior and dopamine signaling in zebrafish (Danio rerio). Environmental Toxicology and Pharmacology. 93:103885.
Abstract
Di (2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer, also known as a developmental toxicant, but its neurobehavioral toxicity remains elusive. This study evaluated the neurobehavioral toxicity and its possible mechanism in larval zebrafish. Embryos at gastrula period (~ 6hour post fertilization, hpf) were exposure to DEHP (0, 1, 2.5, 5 and 10mg/L) for 7 days. Spontaneous tail movement in embryos and swimming activity in larvae were monitored. Alterations in the mRNA expression of genes involved in dopamine signaling and apoptosis pathway were assessed. In situ apoptotic cells were assessed by Acridine orange staining, and oxidative damage were measured using enzymatic assay. The behavior results showed that DEHP inhibited spontaneous tail movement and decreased locomotor activities in the light/dark behavioral test. Meanwhile, behavioral changes were accompanied by increased apoptosis and malondialdehyde (MDA) content, decreased superoxide dismutase (SOD) activity and dopamine (DA) content, and perturbed the expression of genes associated with the synthesis (th), reuptake (dat) and metabolism (mao) of DA, with dopamine receptors (DRs), and with the apoptosis pathway (p53, bax, bcl2, caspase-3, caspase-8, caspase-9). The findings will help to illuminate the possible neurobehavioral toxicity mechanisms of organism exposure to DEHP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping