PUBLICATION
Cell clusters containing intestinal stem cells line, the zebrafish intestine intervillus pocket
- Authors
- Tavakoli, S., Zhu, S., Matsudaira, P.
- ID
- ZDB-PUB-220520-3
- Date
- 2022
- Source
- iScience 25: 104280 (Journal)
- Registered Authors
- Keywords
- Biological sciences, Cell biology, Stem cells research
- MeSH Terms
- none
- PubMed
- 35586068 Full text @ iScience
Citation
Tavakoli, S., Zhu, S., Matsudaira, P. (2022) Cell clusters containing intestinal stem cells line, the zebrafish intestine intervillus pocket. iScience. 25:104280.
Abstract
In the mammalian intestine, stem cells (ISCs) replicate in basal crypts, translocate along the villus, and undergo cell death. This pattern of renewal occurs in the zebrafish intestine in which villi are elongated into villar ridges (VR) separated by intervillus pockets (IVP) but lack the infolded crypts. To understand how epithelial dynamics is maintained without crypts, we investigated the origin of epithelial lineage patterns derived from ISCs in the IVP of chimeric and zebrabow recombinant intestines. We found that the VR epithelium and IVP express the same recombinant colors when expression is under the control of ISC marker promoter prmt1. The expression originates from cell clusters that line the IVP and contain epithelial cells including Prmt1-labeled cells. Our data suggest that Prmt1 is a zebrafish ISC marker and the ISCs reside within basal cell clusters that are functionally analogous to crypts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping