PUBLICATION

Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes

Authors

Vicencio, J., Sánchez-Bolaños, C., Moreno-Sánchez, I., Brena, D., Vejnar, C.E., Kukhtar, D., Ruiz-López, M., Cots-Ponjoan, M., Rubio, A., Melero, N.R., Crespo-Cuadrado, J., Carolis, C., Pérez-Pulido, A.J., Giráldez, A.J., Kleinstiver, B.P., Cerón, J., Moreno-Mateos, M.A.

ID

ZDB-PUB-220514-22

Date

2022

Source

Nature communications 13: 2601 (Journal)

Registered Authors

Vejnar, Charles

Keywords

none

MeSH Terms

Gene Editing*/methods
CRISPR-Associated Protein 9*/genetics
CRISPR-Associated Protein 9*/metabolism
RNA, Messenger
Animals
Zebrafish/genetics
Zebrafish/metabolism
Caenorhabditis elegans/genetics
Caenorhabditis elegans/metabolism
RNA, Guide, Kinetoplastida/genetics
CRISPR-Cas Systems/genetics

PubMed

35552388 Full text @ Nat. Commun.

Abstract

The requirement for Cas nucleases to recognize a specific PAM is a major restriction for genome editing. SpCas9 variants SpG and SpRY, recognizing NGN and NRN PAMs, respectively, have contributed to increase the number of editable genomic sites in cell cultures and plants. However, their use has not been demonstrated in animals. Here we study the nuclease activity of SpG and SpRY by targeting 40 sites in zebrafish and C. elegans. Delivered as mRNA-gRNA or ribonucleoprotein (RNP) complexes, SpG and SpRY were able to induce mutations in vivo, albeit at a lower rate than SpCas9 in equivalent formulations. This lower activity was overcome by optimizing mRNA-gRNA or RNP concentration, leading to mutagenesis at regions inaccessible to SpCas9. We also found that the CRISPRscan algorithm could help to predict SpG and SpRY targets with high activity in vivo. Finally, we applied SpG and SpRY to generate knock-ins by homology-directed repair. Altogether, our results expand the CRISPR-Cas targeting genomic landscape in animals.

Genes / Markers

Figures