PUBLICATION

Context-dependent effects of inflammation on retina regeneration

Authors
Zhou, C., Zhang, X., Chen, Y., Lin, Z., Zhang, S., Zhang, Z., Cao, L., Guan, H., Lu, J., Xu, H.
ID
ZDB-PUB-220512-2
Date
2022
Source
Molecular neurobiology   59(7): 4351-4367 (Journal)
Registered Authors
Xu, Hui, Zhang, Shuqiang, Zhang, Zhiqiang
Keywords
Inflammation, Müller glia, Retina regeneration, Zebrafish
MeSH Terms
  • Animals
  • Cell Proliferation
  • Inflammation
  • Insulins*/pharmacology
  • Mammals
  • N-Methylaspartate/pharmacology
  • Nerve Regeneration/physiology
  • Neuroglia
  • Retina
  • Zebrafish*
PubMed
35538305 Full text @ Mol. Neurobiol.
Abstract
Inflammation is required for the proliferation of Müller glia (MG) into multipotent progenitors (MGPCs) in the injured fish and avian retinas. However, its function in retina regeneration has not been fully understood. Here we investigated the role of inflammation in three different retinal regeneration paradigms in zebrafish (stab-injury, NMDA-injury and insulin treatment). We first show that different types of immune cells and levels of inflammatory cytokines were found in the retinas of these paradigms. Though zymosan injection alone was insufficient to induce MG proliferation in the uninjured retina, immune suppression significantly inhibited MGPC formation in all three paradigms. Enhancing inflammation promoted MGPC formation after stab-injury, while exhibiting a context-dependent role in the NMDA or insulin models. We further show that proper levels of inflammation promoted MG reprogramming and cell cycle re-entry after stab- or NMDA-injury, but excessive inflammation also suppressed MG proliferation in the latter model. Finally, inflammation differentially affected neuronal regeneration in various injury paradigms. Our study reveals the complex and context-dependent role of inflammation during retinal repair in fish and suggests accurate inflammation management may be crucial for successful retina regeneration in mammals.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes