PUBLICATION
Environmentally relevant Manganese concentrations evoke anxiety phenotypes in adult zebrafish
- Authors
- Ferreira, S.A., Loreto, J.S., Dos Santos, M.M., Barbosa, N.V.
- ID
- ZDB-PUB-220507-23
- Date
- 2022
- Source
- Environmental Toxicology and Pharmacology 93: 103870 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Manganese chloride, acetylcholinesterase, anxiety, cortisol
- MeSH Terms
-
- Behavior, Animal
- Animals
- Zebrafish*/physiology
- Anxiety/chemically induced
- Acetylcholinesterase
- Manganese*/toxicity
- Hydrocortisone
- Phenotype
- PubMed
- 35523392 Full text @ Environ. Toxicol. Pharmacol.
Citation
Ferreira, S.A., Loreto, J.S., Dos Santos, M.M., Barbosa, N.V. (2022) Environmentally relevant Manganese concentrations evoke anxiety phenotypes in adult zebrafish. Environmental Toxicology and Pharmacology. 93:103870.
Abstract
Manganese (Mn) is an essential metal for living organisms. However, the excess of Mn can be toxic, especially for the central nervous system. Herein, we used adult zebrafish as model organism to investigate the relationship of an environmentally relevant Mn exposure with the onset of neurobehavioral disturbances and brain biochemical alterations. Fish were exposed to MnCl2 at 0.5, 2.0, 7.5 and 15.0mg/L for 96h, and after submitted to trials for examining exploratory, locomotor and anxiety-related behaviors. The neurobehavioral parameters were followed by the analyses of cell viability, Mn accumulation and acetylcholinesterase activity in the brain, and whole-body cortisol levels. By Novel tank, Light dark and Social preference test, we found that the exposure to Mn, along with locomotor deficits induced anxiety-like phenotypes in zebrafish. Most of these behavioral changes were evoked by the highest concentrations, which also caused cell viability loss, higher accumulation of Mn and increased AChE activity in the brain, and an increase in the whole-body cortisol content. Our findings demonstrated that zebrafish are quite sensitive to levels of Mn found in the environment, and that the magnitude of the neurotoxic effects may be associated with the levels of manganese accumulated in the brain. Interestingly, we showed that Mn exposure in addition to motor deficits may also cause psychiatric abnormalities, namely anxiety.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping