PUBLICATION

β-Ionone causes endocrine disruption, hyperpigmentation and hypoactivity in zebrafish early life stages

Authors
Zhou, W., Wang, Y., Wang, J., Peng, C., Wang, Z., Qin, H., Li, G., Li, D.
ID
ZDB-PUB-220426-3
Date
2022
Source
The Science of the total environment   834: 155433 (Journal)
Registered Authors
Keywords
Locomotion, Melanin, Taste and odor, Toxicity
MeSH Terms
  • Animals
  • Ecosystem
  • Embryo, Nonmammalian
  • Hyperpigmentation*
  • Larva
  • Norisoprenoids
  • Water Pollutants, Chemical*/metabolism
  • Zebrafish/physiology
PubMed
35461947 Full text @ Sci. Total Environ.
Abstract
In nature, the odorous substance β-ionone has been widely detected in aquatic ecosystems. However, little is known about its ecotoxicological effects on freshwater vertebrates. In this study, we aimed to assess the acute toxicity of β-ionone in zebrafish (Danio rerio) embryos from 2 to 120 h post fertilization (hpf) and investigate embryo development, locomotor behavior and pigmentation under different concentrations. The results showed that exposure to β-ionone had an acute toxicity to early life stages of zebrafish and induced a decrease in hatching rate and an increase in the mortality and malformation rate. The median lethal concentration (LC50) of β-ionone at 96 h was observed as 1321 μg/L. In addition, β-ionone not only affected the body length of zebrafish larvae but also regulated the transcription of genes and the levels of hormones involved in the growth hormone/insulin-like growth factor (GH/IGF) and the hypothalamic-pituitary-thyroid (HPT) axes. Moreover, exposure to β-ionone induced significant decreases in locomotor activity and catecholamine neurotransmitters levels. Furthermore, β-ionone stimulated pigmentation via regulation of tyrosinase activity and melanin-related gene expression. Overall, this research could provide new insights into the potential risk of odorants to aquatic organisms.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping