PUBLICATION
A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system
- Authors
- Habicher, J., Manuel, R., Pedroni, A., Ferebee, C., Ampatzis, K., Boije, H.
- ID
- ZDB-PUB-220419-8
- Date
- 2022
- Source
- Gene expression patterns : GEP 44: 119246 (Journal)
- Registered Authors
- Boije, Henrik, Habicher, Judith, Manuel, Remy
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cadherins/genetics
- Cadherins/metabolism
- Central Nervous System/metabolism
- Motor Neurons/metabolism
- Protocadherins*
- Spinal Cord/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 35427788 Full text @ Gene Expr. Patterns
Citation
Habicher, J., Manuel, R., Pedroni, A., Ferebee, C., Ampatzis, K., Boije, H. (2022) A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system. Gene expression patterns : GEP. 44:119246.
Abstract
The wiring of neuronal networks is far from understood. One outstanding question is how neurons of different types link up to form subnetworks within the greater context. Cadherins have been suggested to create an inclusion code where interconnected neurons express the same subtypes. Here, we have used a CRISPR/Cas9 knock-in approach to generate a transgenic zebrafish reporter line for protocadherin 9 (pcdh9), which is predominantly expressed within the central nervous system. Expression of eGFP was detected in subsets of neurons in the cerebellum, retina and spinal cord, in both larvae and juveniles. A closer characterization of the spinal locomotor network revealed that a portion of distinct classes of both excitatory and inhibitory interneurons, as well as motor neurons, expressed pcdh9. This transgenic line could thus be used to test the cadherin network hypothesis, through electrophysiological characterization of eGFP positive cells, to show if these are synaptically connected and form a discrete network within the spinal cord.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping