PUBLICATION
Metabolomic navigated Citrus waste repurposing to restore amino acids disorder in neural lesion
- Authors
- Zhang, X.Y., Zhu, Z.J., Ren, S.P., Deng, Y.C., Xu, J.Y., Zhang, S.M., Gao, J.M., Zhang, Q.
- ID
- ZDB-PUB-220419-17
- Date
- 2022
- Source
- Food Chemistry 387: 132933 (Journal)
- Registered Authors
- Keywords
- Anti-epilepsy, Citrus waste, Food repurposing, Metabolome, Neuroprotection
- MeSH Terms
-
- Amino Acids/analysis
- Animals
- Citrus*/chemistry
- Fruit/chemistry
- Limonins*/analysis
- Refuse Disposal*
- Tandem Mass Spectrometry
- Zebrafish/metabolism
- PubMed
- 35429936 Full text @ Food Chem.
Citation
Zhang, X.Y., Zhu, Z.J., Ren, S.P., Deng, Y.C., Xu, J.Y., Zhang, S.M., Gao, J.M., Zhang, Q. (2022) Metabolomic navigated Citrus waste repurposing to restore amino acids disorder in neural lesion. Food Chemistry. 387:132933.
Abstract
The fruit juice food industry produces huge waste annually, mainly Citrus peel and seeds. We investigated their chemical composition using hydrophilic interaction chromatography (HILIC-) and reverse phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS), revealing 277 compounds, mainly containing flavonoids and limonoids. As the primary representative component in Citrus waste, limonin was selected to be explored new bio-functions. We applied Zebrafish larvae to study the metabolomic response invoked by limonin. The differential metabolites (DMs) varied depending on the exposing concentration of limonin. Enrichment analysis indicated that the identified DMs related to inflammation and neurologic disorders, including epilepsy which were newly discovered for limonoids and Citrus waste. Limonin was found to restore amino acids disorder to take neuroprotection against epilepsy. Our findings provided a new bio-function and purpose for Citrus waste and limonoids. Also, we demonstrated a concise case to repurpose food waste for new applications under metabolome investigation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping