PUBLICATION
A novel cysteine fluorescent probe with large stokes shift for imaging in living cells, zebrafish and living mice
- Authors
- Guo, R., Liu, Q., Tang, Y., Cai, S., Li, X., Gong, X., Yang, Y., Lin, W.
- ID
- ZDB-PUB-220419-16
- Date
- 2022
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 276: 121230 (Journal)
- Registered Authors
- Keywords
- Biological thiols, Cysteine, Fluorescent probe, Large Stokes shift
- MeSH Terms
-
- Glutathione
- Mice
- Sulfhydryl Compounds
- Fluorescent Dyes*
- Animals
- Optical Imaging/methods
- Cysteine*
- Zebrafish
- PubMed
- 35429860 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Guo, R., Liu, Q., Tang, Y., Cai, S., Li, X., Gong, X., Yang, Y., Lin, W. (2022) A novel cysteine fluorescent probe with large stokes shift for imaging in living cells, zebrafish and living mice. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 276:121230.
Abstract
The small molecule biological thiols, such as Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play crucial roles in maintaining various cellular vital activities. In the organism, abnormal levels of small-molecule biological thiols have been associated with a variety of diseases. Therefore, quantitative determination of biological thiols, especially Cys, is significant for understanding their functions in various biological processes. Thus, in this work we designed a new fluorescent probe Ty-Cys1 with a large Stokes shift of 207 nm to monitor Cysteine. The maximum absorption wavelength of Ty-Cys1 was 418 nm, and the maximum emission wavelength was 625 nm. Significantly, the novel probe Ty-Cys1 was effectively in detecting of Cys changes in living cells, zebrafishes, and living mice.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping