PUBLICATION
Identification and characterization of the c-type lysozyme gene from a marine fish, Bostrychus sinensis
- Authors
- Shen, B., Wei, K., Yang, J., Jing, F., Zhang, J.
- ID
- ZDB-PUB-220401-18
- Date
- 2021
- Source
- Developmental and comparative immunology 125: 104232 (Journal)
- Registered Authors
- Keywords
- Antibacterial activity, Chicken-type lysozyme, Chinese black sleeper, Gene expression, Innate immunity
- MeSH Terms
-
- Animals
- Zebrafish Proteins/genetics
- Fishes/immunology*
- Immunity, Innate
- Vibrio parahaemolyticus/physiology*
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Amino Acid Sequence
- Vibrio Infections/immunology*
- Sequence Alignment
- Cloning, Molecular
- Anti-Bacterial Agents
- Muramidase/genetics*
- Muramidase/metabolism
- Up-Regulation
- PubMed
- 34400224 Full text @ Dev. Comp. Immunol.
Citation
Shen, B., Wei, K., Yang, J., Jing, F., Zhang, J. (2021) Identification and characterization of the c-type lysozyme gene from a marine fish, Bostrychus sinensis. Developmental and comparative immunology. 125:104232.
Abstract
In this study, a c-type lysozyme gene (BsLyzC) was identified and characterized from a marine fish, Bostrychus sinensis. The BsLyzC encodes 154 amino acids and contains a signal peptide of 17 amino acids, two catalytic residues and eight cysteine residues. The genomic DNA of BsLyzC consists of four exons and three introns. The BsLyzC shares high sequence similarity with c-type lysozyme from other fish species. The qPCR assays indicated that the BsLyzC exhibited a constitutive expression pattern in eleven examined tissues of healthy B. sinensis individuals. The transcripts of BsLyzC could be significantly induced after infection of Vibrio parahemolyticus in blood, spleen and head kidney. The optimal temperature and pH for recombinant BsLyzC (rBsLyzC) were found to be 50 °C and 6.0, respectively. The rBsLyzC exhibited antibacterial activities against two Gram-positive bacteria and two Gram-negative bacteria. These results indicate that the BsLyzC is involved in the antibacterial immunity of B. sinensis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping