PUBLICATION
Toxicological impacts of nanopolystyrene on zebrafish oocyte with insight into the mechanism of action: An expression-based analysis
- Authors
- Chatterjee, A., Maity, S., Banerjee, S., Dutta, S., Adhikari, M., Guchhait, R., Biswas, C., De, S., Pramanick, K.
- ID
- ZDB-PUB-220329-10
- Date
- 2022
- Source
- The Science of the total environment 830: 154796 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Gene expression, Immunotoxicity, Nanopolystyrene, Oocyte, Oxidative-stress, Zebrafish
- MeSH Terms
-
- Animals
- Microplastics
- Oocytes/metabolism
- Oxidative Stress
- Plastics*/metabolism
- Zebrafish*/metabolism
- PubMed
- 35341844 Full text @ Sci. Total Environ.
Citation
Chatterjee, A., Maity, S., Banerjee, S., Dutta, S., Adhikari, M., Guchhait, R., Biswas, C., De, S., Pramanick, K. (2022) Toxicological impacts of nanopolystyrene on zebrafish oocyte with insight into the mechanism of action: An expression-based analysis. The Science of the total environment. 830:154796.
Abstract
Many studies have investigated the negative impacts of microplastics on teleost fishes with very little or no evidence of their mechanism of action. This scenario entreats us to investigate the toxicities of nanopolystyrene in zebrafish oocyte with emphasis on the mechanism of action. In the present study, the cellular levels of mRNA transcripts of different genetic markers (such as: sod, gpx, nrf2, inos, ucp2, and atp6 (redox-sensitive markers); nfkβ, tnfα, il-10, ikβ, gdf9, and bmp15 (immune markers); gadd45, rad51, p53 and bcl2 (DNA damage and apoptotic)) have been quantified by real-time PCR after 6 h of incubation of isolated oocyte with different doses of nanopolystyrene viz. P0 (control i.e. no polystyrene in culture medium), P1 (100 ng/ml), and P2 (400 ng/ml). Results showed that both the treatment concentrations of nanopolystyrene induce oxidative stress with % DPPH = 30.75, 31.61, and 32.43% for P0, P1, and P2, respectively. Increase in oxidative stress in oocytes with increasing doses of nanopolystyrene was also observed in TBARS assay with MDA content 0.12 and 0.21 μM for P1 and P2, respectively as compaired to the control 0.08 μM. This increased oxidative stress can regulate the expression pattern (upregulation/downregulation) of selected genes leading to different toxic effects like - oxidative stress, immunotoxicity, and apoptosis in oocytes, which suggests the impairment of reproductive functions by nanopolystyrene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping