PUBLICATION
Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy
- Authors
- Kilroy, E.A., Ignacz, A.C., Brann, K.L., Schaffer, C.E., Varney, D., Alrowaished, S.S., Silknitter, K.J., Miner, J.N., Almaghasilah, A., Spellen, T.L., Lewis, A.D., Tilbury, K., King, B.L., Kelley, J.B., Henry, C.A.
- ID
- ZDB-PUB-220325-8
- Date
- 2022
- Source
- eLIFE 11: (Journal)
- Registered Authors
- Henry, Clarissa A.
- Keywords
- developmental biology, dystroglycanopathies, neuromuscular development, skeletal muscle, zebrafish
- Datasets
- GEO:GSE155465
- MeSH Terms
-
- Animals
- Electric Stimulation
- Humans
- Muscle, Skeletal/physiology
- Muscular Dystrophy, Duchenne*/genetics
- Muscular Dystrophy, Duchenne*/therapy
- Neuromuscular Junction/physiology
- Zebrafish
- PubMed
- 35324428 Full text @ Elife
Citation
Kilroy, E.A., Ignacz, A.C., Brann, K.L., Schaffer, C.E., Varney, D., Alrowaished, S.S., Silknitter, K.J., Miner, J.N., Almaghasilah, A., Spellen, T.L., Lewis, A.D., Tilbury, K., King, B.L., Kelley, J.B., Henry, C.A. (2022) Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy. eLIFE. 11:.
Abstract
Neuromuscular electrical stimulation (NMES) allows activation of muscle fibers in the absence of voluntary force generation. NMES could have the potential to promote muscle homeostasis in the context of muscle disease, but the impacts of NMES on diseased muscle are not well understood. We used the zebrafish Duchenne muscular dystrophy (dmd) mutant and a longitudinal design to elucidate the consequences of NMES on muscle health. We designed four neuromuscular stimulation paradigms loosely based on weightlifting regimens. Each paradigm differentially affected neuromuscular structure, function, and survival. Only endurance neuromuscular stimulation (eNMES) improved all outcome measures. We found that eNMES improves muscle and neuromuscular junction morphology, swimming, and survival. Heme oxygenase and integrin alpha7 are required for eNMES-mediated improvement. Our data indicate that neuromuscular stimulation can be beneficial, suggesting that the right type of activity may benefit patients with muscle disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping