PUBLICATION
An assessment of vaping-induced inflammation and toxicity: A feasibility study using a 2-stage zebrafish and mouse platform
- Authors
- Onyenwoke, R.U., Leung, T., Huang, X., Parker, D., Shipman, J.G., Alhadyan, S.K., Sivaraman, V.
- ID
- ZDB-PUB-220324-6
- Date
- 2022
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 163: 112923 (Journal)
- Registered Authors
- Leung, Tin Chung
- Keywords
- C57BL/6J, Electronic cigarette (E-cig), Inflammation, Safety profile, Zebrafish, e-liquids
- MeSH Terms
-
- Animals
- Electronic Nicotine Delivery Systems*
- Feasibility Studies
- Inflammation/chemically induced
- Mice
- Mice, Inbred C57BL
- Pneumonia*/chemically induced
- Vaping*/adverse effects
- Zebrafish
- PubMed
- 35318090 Full text @ Food Chem. Toxicol.
Citation
Onyenwoke, R.U., Leung, T., Huang, X., Parker, D., Shipman, J.G., Alhadyan, S.K., Sivaraman, V. (2022) An assessment of vaping-induced inflammation and toxicity: A feasibility study using a 2-stage zebrafish and mouse platform. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 163:112923.
Abstract
It is currently understood that tobacco smoking is a major cause of pulmonary disease due to pulmonary/lung inflammation. However, due to a highly dynamic market place and an abundance of diverse products, less is known about the effects of e-cigarette (E-cig) use on the lung. In addition, varieties of E-cig liquids (e-liquids), which deliver nicotine and numerous flavor chemicals into the lungs, now number in the 1000s. Thus, a critical need exists for safety evaluations of these E-cig products. Herein, we employed a "2-stage in vivo screening platform" (zebrafish to mouse) to assess the safety profiles of e-liquids. Using the zebrafish, we collected embryo survival data after e-liquid exposure as well as neutrophil migration data, a key hallmark for a pro-inflammatory response. Our data indicate that certain e-liquids induce an inflammatory response in our zebrafish model and that e-liquid exposure alone results in pro-inflammatory lung responses in our C57BL/6J model, data collected from lung staining and ELISA analysis, respectively, in the mouse. Thus, our platform can be used as an initial assessment to ascertain the safety profiles of e-liquid using acute inflammatory responses (zebrafish, Stage 1) as our initial metric followed by chronic studies (C57BL/6J, Stage 2).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping