PUBLICATION
Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish
- Authors
- Lelieveld, L.T., Gerhardt, S., Maas, S., Zwiers, K.C., de Wit, C., Beijk, E.H., Ferraz, M.J., Artola, M., Meijer, A.H., Tudorache, C., Salvatori, D., Boot, R.G., Aerts, J.M.F.G.
- ID
- ZDB-PUB-220323-35
- Date
- 2022
- Source
- Journal of Lipid Research 63(5): 100199 (Journal)
- Registered Authors
- Meijer, Annemarie H.
- Keywords
- Gaucher disease, acid ceramidase, dopaminergic neurons, glucosylceramide, lipid metabolism, lysosphingolipids, sphingolipids, zebrafish
- MeSH Terms
-
- Acid Ceramidase
- Animals
- Gaucher Disease*/genetics
- Glucosylceramidase/genetics
- Glucosylceramidase/metabolism*
- Glucosylceramides
- Humans
- Psychosine/analogs & derivatives
- Zebrafish/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 35315333 Full text @ J. Lipid Res.
Citation
Lelieveld, L.T., Gerhardt, S., Maas, S., Zwiers, K.C., de Wit, C., Beijk, E.H., Ferraz, M.J., Artola, M., Meijer, A.H., Tudorache, C., Salvatori, D., Boot, R.G., Aerts, J.M.F.G. (2022) Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish. Journal of Lipid Research. 63(5):100199.
Abstract
In Gaucher disease (GD), the deficiency of glucocerebrosidase (GCase/ GBA1) causes lysosomal accumulation of glucosylceramide (GlcCer), which is partly converted by acid ceramidase (ACase) to glucosylsphingosine (GlcSph) in the lysosome. Chronically elevated blood and tissue GlcSph is thought to contribute to symptoms in GD patients as well as to increased risk for Parkinson's disease. On the other hand, formation of GlcSph may be beneficial since the water soluble sphingoid base is excreted via urine and bile. To study the role of excessive GlcSph formation during glucocerebrosidase deficiency we studied zebrafish that have two orthologues of acid ceramidase, Asah1a and Asah1b. Only the latter is involved in formation of GlcSph in glucocerebrosidase-deficient zebrafish as revealed by knockouts of Asah1a or Asah1b with GCase deficiency (either pharmacologically induced or genetic). Comparison of zebrafish with excessive GlcSph (gba1-/- fish) and without GlcSph (gba1-/-:asah1b-/- fish) allowed us to study consequences of chronic high levels of GlcSph. Prevention of excessive GlcSph in gba1-/-:asah1b-/- fish did not restrict storage cells, GlcCer accumulation or neuroinflammation. However, GD fish lacking excessive GlcSph show an ameliorated course of disease reflected by significantly increased lifespan, delayed locomotor abnormality, and delayed development of an abnormal curved back posture. The loss of tyrosine hydroxylase 1 (th1) mRNA, a marker of dopaminergic neurons, is slowed down in brain of GD fish lacking excessive GlcSph. In conclusion, in the zebrafish GD model, excess GlcSph has little impact on (neuro)inflammation or the presence of GlcCer-laden macrophages, but rather seems harmful to th1-positive dopaminergic neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping