PUBLICATION
Liver and muscle-specific effects of phoenixin-20 on the insulin-like growth factor system mRNAs in zebrafish
- Authors
- Rajeswari, J.J., Vélez, E.J., Unniappan, S.
- ID
- ZDB-PUB-220320-11
- Date
- 2022
- Source
- Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society 63: 101456 (Journal)
- Registered Authors
- Unniappan, Suraj
- Keywords
- Growth, Igfs, Liver, Muscle, Phoenixin-20, Smim20, Zebrafish, siRNA
- MeSH Terms
-
- Animals
- Female
- Insulin-Like Growth Factor Binding Proteins/metabolism
- Insulin-Like Growth Factor I/genetics
- Insulin-Like Growth Factor I/metabolism
- Liver/metabolism
- Male
- Mammals/genetics
- Mammals/metabolism
- Muscles/metabolism
- Peptide Hormones
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Receptors, Somatomedin/metabolism
- Somatomedins*/genetics
- Somatomedins*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 35305530 Full text @ Growth Horm IGF Res
Citation
Rajeswari, J.J., Vélez, E.J., Unniappan, S. (2022) Liver and muscle-specific effects of phoenixin-20 on the insulin-like growth factor system mRNAs in zebrafish. Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society. 63:101456.
Abstract
Objective Phoenixin-20 (Pnx-20) is a bioactive peptide with endocrine-like actions in vertebrates. Recent studies suggest Pnx-20 promotes growth hormone/insulin-like growth factors (Gh/Igf) axis, an important endocrine regulator of growth in mammals and fish.
Design In this research, we determined whether Pnx-20 affects the different members of the Igf family, its binding proteins and receptors (Igf-system) in zebrafish liver and muscle.
Results In vivo administration of Pnx-20 downregulated igfs, igf receptors (igfrs) and igf binding protein (igfbp) 5 mRNA expression in the liver of male and female zebrafish at both 1 and 6 h post-intraperitoneal (IP) injection. Interestingly, this effect occurred at a relatively earlier timepoint in female zebrafish suggesting sex-specific differences in Pnx-20 action. Besides, either 6 or 24 h in vitro incubations with Pnx-20 downregulated the expression of all igfs, igfrs and igfbp5 mRNAs (except igf2a) analyzed in a zebrafish liver cell (ZFL) line. Moreover, siRNA-mediated knockdown of Pnx-20 upregulated all Igf-system mRNAs analyzed in ZFL cells. Together, these results (both in vivo and in vitro) revealed a general suppressive action for both endogenous and exogenous Pnx-20 on the hepatic Igf-system of zebrafish. In contrast, a general sex-specific upregulation of the Igf-system mRNAs analyzed was found in the muscle of Pnx-20 injected fish. Future research should explore the sex- and time-differences observed in the present study.
Conclusions Collectively, this research shows that Pnx-20 is a tissue-specific regulator of the liver (suppressor) and muscle (stimulant) Igf signaling in both male and female zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping