PUBLICATION

Interaction of transcription factors Islet2 and Nr2f1b to control vascular patterning during zebrafish development

Authors
Wang, Y.S., Huang, Y.S., Chiu, C.C., Wu, T.Y., Zhou, J.Q., Liang, S.R., Tai, M.H., Wu, C.Y.
ID
ZDB-PUB-220319-9
Date
2022
Source
Biochemical and Biophysical Research Communications   604: 123-129 (Journal)
Registered Authors
Keywords
Intersegmental vessel, Islet2, Nr2f1b, Transcription factors, Vascular development and zebrafish
MeSH Terms
  • LIM-Homeodomain Proteins*/genetics
  • LIM-Homeodomain Proteins*/metabolism
  • Arteries*/growth & development
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Animals
  • Transcription Factors*/genetics
  • Transcription Factors*/metabolism
  • Veins
  • Zebrafish*/genetics
  • Zebrafish*/growth & development
(all 11)
PubMed
35303678 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Many regulators controlling arterial identity are well described; however, transcription factors that promote vein identity and vascular patterning have remained largely unknown. We previously identified the transcription factors Islet2 (Isl2) and Nr2f1b required for specification of the vein and tip cell identity mediated by notch pathway in zebrafish. However, the interaction between Isl2 and Nr2f1b is not known. In this study, we report that Nr2f2 plays minor roles on vein and intersegmental vessels (ISV) growth and dissect the genetic interactions among the three transcription factors Isl2, Nr2f1b, and Nr2f2 using a combinatorial knockdown strategy. The double knockdown of isl2/nr2f1b, isl2/nr2f2, and nr2f1b/nr2f2 showed the enhanced defects in vasculature including less completed ISV, reduced veins, and ISV cells. We further tested the genetic relationship among these three transcription factors. We found isl2 can regulate the expression of nr2f1b and nr2f2, suggesting a model where Isl2 functions upstream of Nr2f1b and Nr2f2. We hypothsized that Isl2 and Nr2f1b can function together through cis-regulatory binding motifs. In-vitro luciferase assay results, we showed that Isl2 and Nr2f1b can cooperatively enhance gene expression. Moreover, co-immunoprecipitation results indicated that Isl2 and Nr2f1b interact physically. Together, we showed that the interaction of the Nr2f1b and Nr2f2 transcription factors in combination with the Islet2 play coordinated roles in the vascular development of zebrafish.
Genes / Markers
Figures
No images available
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci5TgTransgenic Insertion
    la116TgTransgenic Insertion
      sd2TgTransgenic Insertion
        y7TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          Sequence Targeting Reagents
          Fish
          Antibodies
          Orthology
          Engineered Foreign Genes
          Marker Marker Type Name
          DsRedEFGDsRed
          EGFPEFGEGFP
          GFPEFGGFP
          mCherryEFGmCherry
          1 - 4 of 4
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          Mapping
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          Citation
          Wang, Y.S., Huang, Y.S., Chiu, C.C., Wu, T.Y., Zhou, J.Q., Liang, S.R., Tai, M.H., Wu, C.Y. (2022) Interaction of transcription factors Islet2 and Nr2f1b to control vascular patterning during zebrafish development. Biochemical and Biophysical Research Communications. 604:123-129.