PUBLICATION
Mutation of spexin2 promotes feeding, somatic growth, adiposity and insulin resistance in zebrafish
- Authors
- Zhao, T., Ye, Z., Liu, Y., Lin, H., Li, S., Zhang, Y.
- ID
- ZDB-PUB-220317-5
- Date
- 2022
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 322(5): R454-R465 (Journal)
- Registered Authors
- Keywords
- energy metabolic regulation, satiety factor, spexin2, zebrafish
- MeSH Terms
-
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Animals
- Amino Acids/metabolism
- Adiposity/genetics
- Mutation
- Male
- Hypothalamus/metabolism
- Insulin Resistance*/genetics
- PubMed
- 35293264 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Zhao, T., Ye, Z., Liu, Y., Lin, H., Li, S., Zhang, Y. (2022) Mutation of spexin2 promotes feeding, somatic growth, adiposity and insulin resistance in zebrafish. American journal of physiology. Regulatory, integrative and comparative physiology. 322(5):R454-R465.
Abstract
Spexin2 (spx2) is a newly identified gene in vertebrates, but its biological functions remain unclear. In this study, we cloned the full-length cDNA of spx2 in zebrafish. The 288 bp open reading frame encodes a protein of 95 amino acids (aa) that contains a 14 aa mature peptide. Spx2 is highly expressed in brain and testis. Its expression was significantly down-regulated in the hypothalamus after a 1 h feeding treatment and 7 days of food deprivation. Using a zebrafish spx2-/- mutant line, we observed a greater amount of food intake and changes in mRNA levels of feeding factors. We found that, SPX2 acts as a satiety factor that inhibits food intake by downregulating the expression of agouti related neuropeptide (agrp). Moreover, spx2 mutant fish exhibited a larger body size, excessive lipid accumulation, and insulin resistance. Taken together, our results revealed that SPX2 functions as a satiety factor involved in energy metabolic regulation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping