PUBLICATION
Zebrafish xenograft model for studying the function of lncRNA SNHG4 in the proliferation and migration of colorectal cancer
- Authors
- Wang, J., Zhang, X.Y., Xu, D.Y.
- ID
- ZDB-PUB-220315-22
- Date
- 2022
- Source
- Journal of gastrointestinal oncology 13: 210-220 (Journal)
- Registered Authors
- Keywords
- Zebrafish xenograft, cell migration, cell proliferation, colorectal cancer (CRC), lncRNA SNHG4
- MeSH Terms
- none
- PubMed
- 35284103 Full text @ J Gastrointest Oncol
Citation
Wang, J., Zhang, X.Y., Xu, D.Y. (2022) Zebrafish xenograft model for studying the function of lncRNA SNHG4 in the proliferation and migration of colorectal cancer. Journal of gastrointestinal oncology. 13:210-220.
Abstract
Background The zebrafish xenograft model has become a reliable in vivo model for human cancer research. Compared to a mouse model, the zebrafish xenograft has many advantages, including optical transparency, intuitive in vivo observation, and speed. Long noncoding RNAs (lncRNAs) have been identified as crucial regulatory factors in the progression of colorectal cancer (CRC). The biological function of lncRNA small nucleolar RNA host gene 4 (SNHG4) in CRC is still unclear.
Methods We analyzed the expression of SNHG4 in CRC patient samples by the Gene Expression Profiling Interactive Analysis (GEPIA) software. The quantitative real time-polymerase chain reaction (qRT-PCR) was used to verify in CRC cell lines. The colony formation assay was used to study the cell proliferation, and we used the transwell assay to detect the migration ability. Then the zebrafish xenograft models were used to confirm these roles of SNHG4in vivo. Moreover, we detected epithelial mesenchymal transition (EMT) related genes by qRT-PCR.
Results We found the expression of SNHG4 was upregulated in CRC patient samples by analyzing GEPIA software, which was also verified in CRC cell lines. We also found that silencing SNHG4 inhibited the proliferation and migration of CRC cells, and its roles were verified in zebrafish xenografts in vivo. Further, we found that the expression of E-cadherin was significantly upregulated and N-cadherin was downregulated when knocking-down SNHG4 in CRC cells.
Conclusions Our findings demonstrated that SNHG4 played oncogenic roles in CRC, which could be a potential target for treatment of CRC patients, and the results strongly revealed that zebrafish xenograft could be used for functional research of lncRNAs in human cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping