PUBLICATION
Identification of Scd5 as a functional regulator of visceral fat deposition and distribution
- Authors
- Zhang, Q., Sun, S., Zhang, Y., Wang, X., Li, Q.
- ID
- ZDB-PUB-220308-14
- Date
- 2022
- Source
- iScience 25: 103916 (Journal)
- Registered Authors
- Li, Qiang, Wang, Xu
- Keywords
- Genetics, Molecular biology, Molecular genetics
- MeSH Terms
- none
- PubMed
- 35252813 Full text @ iScience
Citation
Zhang, Q., Sun, S., Zhang, Y., Wang, X., Li, Q. (2022) Identification of Scd5 as a functional regulator of visceral fat deposition and distribution. iScience. 25:103916.
Abstract
Ectopic deposition of visceral adipose tissue (VAT) in abdomen is usually accompanied with systematic chaos of energy metabolism, a higher risk of cardiovascular diseases and type II diabetes. Here, we identified a previously unexplored gene Scd5 as a master regulator of fat distribution, which alone plays a significant role in determining the VAT accumulation. Firstly, zebrafish scd5 had the highest homology with human SCD5 compared to other SCDs in mouse and rat. We then observed that scd5-homozygous mutant zebrafish displayed a puffy, short and rounded apple-shaped figure. Whole-mount micro-CT scan showed that excessive VAT deposition and short spine are responsible for the abnormal body ratio. And the supplementation of ω3-polyunsaturated fatty acid (ω3-PUFA) in dietary significantly decreased VAT accumulation in scd5-/- zebrafish. Lastly, transcriptional analyses revealed that the Wnt, PPAR, C/EBP, and fat synthesis signaling pathways are significantly affected in the VAT of scd5-/- mutant and restored by ω3-PUFA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping