PUBLICATION
Docetaxel and 5-FU enhanced the inhibitory effects of apatinib and ramucirumab on growth and migration of gastric cancer
- Authors
- Wu, J.Q., Fan, R.Y., Zhai, J., Li, C.Y., Wei, P., Shen, L.Z., He, M.F., Wang, P., Huang, X.E.
- ID
- ZDB-PUB-220303-28
- Date
- 2022
- Source
- Life sciences 296: 120439 (Journal)
- Registered Authors
- Keywords
- Anti-angiogenic therapy, Combined treatment, Gastric cancer, Zebrafish, zPDX
- MeSH Terms
-
- Angiogenesis Inhibitors/administration & dosage
- Angiogenesis Inhibitors/pharmacology
- Animals
- Animals, Genetically Modified
- Antibodies, Monoclonal, Humanized/administration & dosage
- Antineoplastic Combined Chemotherapy Protocols/pharmacology*
- Cell Line, Tumor
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Docetaxel/administration & dosage
- Embryo, Nonmammalian
- Fluorouracil/administration & dosage
- Humans
- Proto-Oncogene Proteins c-akt/metabolism
- Pyridines/administration & dosage
- Stomach Neoplasms/drug therapy*
- Stomach Neoplasms/metabolism
- Stomach Neoplasms/pathology*
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Xenograft Model Antitumor Assays
- Zebrafish/embryology
- Zebrafish/genetics
- PubMed
- 35235851 Full text @ Life Sci.
Citation
Wu, J.Q., Fan, R.Y., Zhai, J., Li, C.Y., Wei, P., Shen, L.Z., He, M.F., Wang, P., Huang, X.E. (2022) Docetaxel and 5-FU enhanced the inhibitory effects of apatinib and ramucirumab on growth and migration of gastric cancer. Life sciences. 296:120439.
Abstract
Background Gastric cancer (GC) is one of the most common malignant tumors in the world. The clinical benefit of anti-angiogenic strategy as a single drug is limited. Some studies showed that the combination of anti-angiogenic therapy and chemotherapy exhibited synergistic effect and reduced the side effects of chemotherapy drugs. We investigated the combined effects of these two types of drugs in gastric cancer cells in vitro and in vivo.
Methods cell viability, migration, invasion, and apoptosis were evaluated by CCK8, wound-healing, transwell, and Annexin V-FITC assay, respectively. In vivo anti-cancer efficacy was tested for the cell proliferation and metastasis in cell line derived tumor xenograft (CDX) model and patient derived tumor xenografted (PDX) model based on Tg (fli-1: EGFP) zebrafish embryos; RESULTS: In the cell experiments, the combination of the two types of drugs could inhibit the proliferation and metastasis of gastric cancer cells and promote apoptosis through VEGFR2/AKT/ERK1/2 signal. In the zebrafish CDX (zCDX) model and zebrafish PDX (zPDX) model, the combination of the two treatment also showed a synergistic effect in inhibiting gastric cancer cell metastasis and cell proliferation.
Conclusions Apatinib/ramucirumab targeted therapy combined with docetaxel or 5-fluorouracil (5-FU) may serve as an effective treatment strategy for patients with advanced gastric cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping