PUBLICATION
Seizures initiate in zones of relative hyperexcitation in a zebrafish epilepsy model
- Authors
- Niemeyer, J.E., Gadamsetty, P., Chun, C., Sylvester, S., Lucas, J.P., Ma, H., Schwartz, T.H., Aksay, E.R.F.
- ID
- ZDB-PUB-220224-7
- Date
- 2022
- Source
- Brain : a journal of neurology 145(7): 2347-2360 (Journal)
- Registered Authors
- Keywords
- E/I balance, calcium imaging, ictogenesis, seizure initiation, seizure propagation
- MeSH Terms
-
- Animals
- Brain
- Epilepsy*
- Neurons
- Seizures
- Zebrafish*
- PubMed
- 35196385 Full text @ Brain
Citation
Niemeyer, J.E., Gadamsetty, P., Chun, C., Sylvester, S., Lucas, J.P., Ma, H., Schwartz, T.H., Aksay, E.R.F. (2022) Seizures initiate in zones of relative hyperexcitation in a zebrafish epilepsy model. Brain : a journal of neurology. 145(7):2347-2360.
Abstract
Seizures are thought to arise from an imbalance of excitatory and inhibitory neuronal activity. While most classical studies suggest excessive excitatory neural activity plays a generative role, some recent findings challenge this view and instead argue that excessive activity in inhibitory neurons initiates seizures. We investigated this question of imbalance in a zebrafish seizure model with two-photon imaging of excitatory and inhibitory neuronal activity throughout the brain using a nuclear-localized calcium sensor. We found that seizures consistently initiated in circumscribed zones of the midbrain before propagating to other brain regions. Excitatory neurons were both more prevalent and more likely to be recruited than inhibitory neurons in initiation as compared with propagation zones. These findings support a mechanistic picture whereby seizures initiate in a region of hyper-excitation, then propagate more broadly once inhibitory restraint in the surround is overcome.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping